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Volume 19, Issue 2 (Summer & Autumn 2022)
ASJ 2022, 19(2): 119-124 |
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Monsef F, Sadeghinezhad J, Bayat A, Bojarzadeh H. Histochemical Study of Intestinal Mucins in Adult Male New Zealand Rabbit. ASJ 2022; 19 (2) :119-124
URL: http://anatomyjournal.ir/article-1-595-en.html
URL: http://anatomyjournal.ir/article-1-595-en.html
1- Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Abstract: (444 Views)
Introduction: The rabbit is an important laboratory species for various experimental studies in the gastrointestinal tract. A detailed knowledge of the histological and chemical structure of the rabbit small intestine is important for identifying pathogens and applying appropriate treatment methods. This study aims to investigate the mucin secreted by goblet cells and Brunner glands in different parts of the rabbit small intestine.
Methods: Five adult male New Zealand rabbits were used for this study. Different parts of the small intestine, including the duodenum, jejunum, and ileum, were collected according to a systematic, uniform random procedure. Samples were processed, embedded in paraffin, and cut into 5-µm sections. Sections were stained for histochemical examination with periodic acid-schiff (PAS) and Alcian blue (AB) (pH 1.0 and 2.5) and PAS-AB (pH 2.5) and aldehyde-fuchsin (AF-AB) (pH 2.5) staining techniques.
Results: The results showed that the goblet cells of the small intestine responded positively to PAS staining, whereas no positive response was observed in the secretory units of Brunner’s glands. The secretory units of goblet cells and Brunner’s glands responded positively to AB at pH 1 and 2.5, representing sulfated and carboxylic acid mucin, respectively. PAS-AB Staining with (pH 2.5) showed that in the goblet cells of the small intestine, the content of neutral mucin was higher than acidic mucin, while the secretory units of Brunner’s glands contained only acidic mucin. In the AF-AB (pH 2.5) staining of the goblet cells, the content of sulfated mucin was higher than that of carboxylate mucin. In Brunner’s glands, the amount of carboxylate mucin was higher than that of sulfate mucin.
Conclusion: In this study, the histochemical mucin characteristics of rabbit small intestine are described in detail. The results of the present study reveal similarities and differences with other mammals.
Methods: Five adult male New Zealand rabbits were used for this study. Different parts of the small intestine, including the duodenum, jejunum, and ileum, were collected according to a systematic, uniform random procedure. Samples were processed, embedded in paraffin, and cut into 5-µm sections. Sections were stained for histochemical examination with periodic acid-schiff (PAS) and Alcian blue (AB) (pH 1.0 and 2.5) and PAS-AB (pH 2.5) and aldehyde-fuchsin (AF-AB) (pH 2.5) staining techniques.
Results: The results showed that the goblet cells of the small intestine responded positively to PAS staining, whereas no positive response was observed in the secretory units of Brunner’s glands. The secretory units of goblet cells and Brunner’s glands responded positively to AB at pH 1 and 2.5, representing sulfated and carboxylic acid mucin, respectively. PAS-AB Staining with (pH 2.5) showed that in the goblet cells of the small intestine, the content of neutral mucin was higher than acidic mucin, while the secretory units of Brunner’s glands contained only acidic mucin. In the AF-AB (pH 2.5) staining of the goblet cells, the content of sulfated mucin was higher than that of carboxylate mucin. In Brunner’s glands, the amount of carboxylate mucin was higher than that of sulfate mucin.
Conclusion: In this study, the histochemical mucin characteristics of rabbit small intestine are described in detail. The results of the present study reveal similarities and differences with other mammals.
Type of Study: Original |
Subject:
Histology
Received: 2022/02/10 | Accepted: 2022/05/23 | Published: 2022/07/1
Received: 2022/02/10 | Accepted: 2022/05/23 | Published: 2022/07/1
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