Fri, Apr 19, 2024
Volume 2, Issue 1 (Spring 2004)
ASJ 2004, 2(1): 29-35 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Joghataei M T, Behzadi Z, Nikbakht F. Effect of Specific Lesion of Non Serotonergic Pathway on Neurons of Nucleus Raphe Magnus Morphology in Rat. ASJ 2004; 2 (1) :29-35
URL: http://anatomyjournal.ir/article-1-360-en.html
URL: http://anatomyjournal.ir/article-1-360-en.html
1- Anatomy Department, Iran Medical Sciences University, Tehran, Iran.
Abstract: (862 Views)
Purpose: The nucleus raphe magnus (NRM) is a medullary nucleus containing serotonergic and non serotonergic neurons, both of which densely project to spinal cord. The goal of this study was to determine the role of these non serotonergic neurons in pain perception and their cytological changes after the specific lesion of bulbo-spinal serotonergic pathway.
Materials and Methods: Male rats were intrathecally administered 5,6 Dihydroxytryptamine (5,6 DHT). Then animals were perfused 5, 14 and 30 days after the injection of neurotoxin or vehicle. Serial microtom sections (5-8 microns) were Nissl Stained, and the cytological changes of neurons were evaluated by light microscope.
Results: The results revealed that the number of all three types of NRM neurons was significantly reduced following the intrathecal injection of 5, 6 DHT. The reduction was not recovered within one month, although; the pain perception returned to normal level. In addition, a significant enlargement (increase in long axis) was also observed in NRM neurons after the chemical lesions of descending 5-HT pathways (p<0.001).
Conclusion: It is concluded that no evident recovery will be seen in serotonergic pathways after their select in destruction, although the pain perception becomes normal after one month. Normal threshold of pain indicates a probable function adaptation of other transmission systems, which compensates for the loss of serotonergic neurons.
Materials and Methods: Male rats were intrathecally administered 5,6 Dihydroxytryptamine (5,6 DHT). Then animals were perfused 5, 14 and 30 days after the injection of neurotoxin or vehicle. Serial microtom sections (5-8 microns) were Nissl Stained, and the cytological changes of neurons were evaluated by light microscope.
Results: The results revealed that the number of all three types of NRM neurons was significantly reduced following the intrathecal injection of 5, 6 DHT. The reduction was not recovered within one month, although; the pain perception returned to normal level. In addition, a significant enlargement (increase in long axis) was also observed in NRM neurons after the chemical lesions of descending 5-HT pathways (p<0.001).
Conclusion: It is concluded that no evident recovery will be seen in serotonergic pathways after their select in destruction, although the pain perception becomes normal after one month. Normal threshold of pain indicates a probable function adaptation of other transmission systems, which compensates for the loss of serotonergic neurons.
Type of Study: Original |
Subject:
Morphometry
Received: 2021/12/25 | Accepted: 2004/04/22 | Published: 2004/04/22
Received: 2021/12/25 | Accepted: 2004/04/22 | Published: 2004/04/22
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Contact Information
Anatomical Sciences Journal (ASJ)
Negah Institute for Scientific Communication, No.15, Na'eemi St., Mirzaye Shirazi St., Tehran, Iran.
Publisher Tel : +9821 4535 5555;
+9821 4535 5000
Website: http://www.anatomyjournal.ir/
E-mail: anatomyjournal@gmail.com
