Thu, May 9, 2024
Volume 1, Issue 4 (Autumn 2003)
ASJ 2003, 1(4): 53-62 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mohammadi M, Allameh A A, Asadi Sar Yazdi M H, KHhajeh M R. Inhibition of Microsome-Mediated Binding of Benzo (Α) Pyrene to "Dna By Cytosolic Reaction From Liver And Skin Rats in Cvitro. ASJ 2003; 1 (4) :53-62
URL: http://anatomyjournal.ir/article-1-341-en.html
URL: http://anatomyjournal.ir/article-1-341-en.html
1- Biochemistry and Microbiology Departmen, Islamic Azad University, Flavarjan, Iran.
Abstract: (751 Views)
Purpose: The aim of this study was to evaluate the effect of age on the capacity of liver and epiderm of adult and weanging rats in transformation of Benzo (α) Pyrene.
Materials and Methods: In a metabolic activiation assay system, cytochorome P-50 (from microsomal fraction) catalyses the formation of reactive epoxide of BaP which can then interact with exogenous DNA The capacity of cytochrome P-450 for BαP-DNA adducts formation from different organs are adult rat live>>> adult rat skin>> weanging rat liver> weanling rat skin. With the addition of cytosol (the source of glutathione S transferase (GST) to the microsome-mediated system an In vitro detoxification model has been established.
Results: The results obtained by the addition of different cytosolic samples are shown in several tables in the text. These data show that isolation of subcellular fractions from young rats are less efficient in the biotransformation of BαP and detoxification by GSH is a passive process that depends on activation process. Also metabolic activation of BaP with cytochorome P-450 is not the first degree with glutathione detoxification. However, the results obtined In vitro do not reflect the exact change In vivo.
Materials and Methods: In a metabolic activiation assay system, cytochorome P-50 (from microsomal fraction) catalyses the formation of reactive epoxide of BaP which can then interact with exogenous DNA The capacity of cytochrome P-450 for BαP-DNA adducts formation from different organs are adult rat live>>> adult rat skin>> weanging rat liver> weanling rat skin. With the addition of cytosol (the source of glutathione S transferase (GST) to the microsome-mediated system an In vitro detoxification model has been established.
Results: The results obtained by the addition of different cytosolic samples are shown in several tables in the text. These data show that isolation of subcellular fractions from young rats are less efficient in the biotransformation of BαP and detoxification by GSH is a passive process that depends on activation process. Also metabolic activation of BaP with cytochorome P-450 is not the first degree with glutathione detoxification. However, the results obtined In vitro do not reflect the exact change In vivo.
Type of Study: Original |
Subject:
Morphometry
Received: 2021/12/25 | Accepted: 2003/10/18 | Published: 2003/10/18
Received: 2021/12/25 | Accepted: 2003/10/18 | Published: 2003/10/18
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Contact Information
Anatomical Sciences Journal (ASJ)
Negah Institute for Scientific Communication, No.15, Na'eemi St., Mirzaye Shirazi St., Tehran, Iran.
Publisher Tel : +9821 4535 5555;
+9821 4535 5000
Website: http://www.anatomyjournal.ir/
E-mail: anatomyjournal@gmail.com
