Volume 17, Issue 2 (Summer & Autumn 2020)                   ASJ 2020, 17(2): 55-62 | Back to browse issues page

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Azari Z, Zamini A, Dabirian S, Mehrdad M, Olfati J, Dadgaran I et al . Cytotoxicity Effect of Hull-Less Seed Pumpkin Extract on Human Papillary Thyroid Cancer Cell Line. ASJ 2020; 17 (2) :55-62
URL: http://anatomyjournal.ir/article-1-222-en.html
1- Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
2- Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.
3- Department of Endocrinology, Guilan University of Medical Sciences, Rasht, Iran.
4- Department of Horticultural, Faculty of Agriculture Science, Guilan University of Medical Sciences, Rasht,Iran.
5- Medical Educational Research Centre, Guilan University of Medical Sciences, Rasht, Iran.
Abstract:   (2481 Views)
Introduction: Thyroid cancer is the most prevalent endocrine malignancy tumor, and its incidence is increasing. Chemotherapy drugs like Paclitaxel (PTX) are a common treatment for cancer; however, they have many adverse effects. Plants are a source of anticancer agents. The present study assessed the cytotoxic effects of hydro-alcoholic extract in comparison with those of paclitaxel on Papillary Thyroid Cancer (PTC) cell line.
Methods: PTC cell line was treated by different concentrations of extract and paclitaxel for 24, 48, and 72 h. Cytotoxicity was examined through Trypan blue and clonogenic assays. Acridine Orange/Ethidium Bromide (AO/EB) staining was used for detecting apoptotic cells. The observations were statistically tabulated and analyzed. 
Results: The Trypan blue staining results suggested that hydro-alcoholic extract had a cytotoxic effect on PTC cells. Our results of AO/EB staining revealed that the hydro-alcoholic extract of Hull-Less Seed Pumpkin (HLSP) in the concentration of ≥100 μl/mL induced significant apoptosis in the PTC cultured cell (P<0.05). In addition, the AO/EB staining data suggested an increase in the number of apoptotic cells with increasing the concentration of extract and paclitaxel. The clonogenic assay results indicated a decrease in colonies by increasing the concentration. Comparing the groups treated by paclitaxel or HLSP extract, with the control group revealed significant differences between them (P<0.05).
Conclusion: The HLSP extract had a cytotoxic effect on the human PTC cell line. Based on the adverse effects of chemotherapy drugs, this extract can be considered as a beneficial agent for PTC treatment.
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Type of Study: Original | Subject: Morphometry
Received: 2018/06/30 | Accepted: 2018/07/5 | Published: 2019/04/20

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