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Volume 20, Issue 2 (Summer & Autumn-In Press 2023)
ASJ 2023, 20(2): 0-0 |
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Gharaei P, Safi S, Hesaraki S, Kakoienejad M M. Biochemical and Histopathological Investigation of Protective effects of Aged Garlic Extract on Heart and Kidney against Injuries Caused by Doxorubicin in Mice. ASJ 2023; 20 (2)
URL: http://anatomyjournal.ir/article-1-629-en.html
URL: http://anatomyjournal.ir/article-1-629-en.html
1- Department of Veterinary Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2- Iran University of Medical Sciences, Tehran, Iran.
2- Iran University of Medical Sciences, Tehran, Iran.
Abstract: (502 Views)
Background: Doxorubicin, a widely used chemotherapeutic agent, is effective against various cancers but its utility is limited by its dose-dependent toxicity, especially its effect on the heart and kidneys. Traditional medicine and food sources have long utilized garlic extract as an anti-inflammatory agent. This study investigates the protective effects of Aged Garlic Extract (AGE) against doxorubicin-induced cardiac and renal toxicity.
Methods: 25 BALB/c mice were randomly divided into 5 equal groups (5 series). Control group: received saline serum (2 ml/kg) by gavage for 10 consecutive days until the end of the study. DOX group: saline serum (2 ml/kg) by gavage for 10 days, plus intraperitoneal injection of a single dose of 15 mg/kg on the 7th day of the study. Group 150 = 150 mg/kg extract is administered by gavage for 10 days, but on the 7th day of the study, a single dose of 15 mg/kg doxorubicin was injected intraperitoneally. Group 300 = 300 mg/kg extract for 10 days, and single dose of 15 mg/kg doxorubicin on the 7th day. Group 600 = 600 mg/kg extract for 10 days, and single dose of 15 mg/kg doxorubicin on the 7th day. Blood sampling was done to measure the serum level of creatinine (creatinine) to evaluate the kidney and creatine kinase (CK) to evaluate the heart muscles along with the evaluation of total oxidant level (TOS) and total antioxidant capacity (TAC). Histopathological examinations were performed for both organs of the heart and kidneys. The lesions were graded from 0 to 9 based on the severity of the lesions.
Results: Doxorubicin administration resulted in significant cardiac and renal damage, evident by macroscopic and microscopic alterations, increased serum creatinine levels, and elevated creatine kinase concentrations. The 150 mg/kg AGE group demonstrated the most pronounced protective effects, reducing tissue damage and enhancing total antioxidant capacity compared to the doxorubicin-only group. Although Higher doses of AGE accompanied higher TAC, they did not confer additional protective benefits and may have compromised renal function.
Conclusion: Aged Garlic Extract effectively mitigates doxorubicin-induced toxicity in cardiac and renal tissues, with a notable dose-dependent response. This research suggests that AGE might be a useful addition to doxorubicin treatment protocols, warranting further investigation into its mechanisms and potential clinical applications.
Methods: 25 BALB/c mice were randomly divided into 5 equal groups (5 series). Control group: received saline serum (2 ml/kg) by gavage for 10 consecutive days until the end of the study. DOX group: saline serum (2 ml/kg) by gavage for 10 days, plus intraperitoneal injection of a single dose of 15 mg/kg on the 7th day of the study. Group 150 = 150 mg/kg extract is administered by gavage for 10 days, but on the 7th day of the study, a single dose of 15 mg/kg doxorubicin was injected intraperitoneally. Group 300 = 300 mg/kg extract for 10 days, and single dose of 15 mg/kg doxorubicin on the 7th day. Group 600 = 600 mg/kg extract for 10 days, and single dose of 15 mg/kg doxorubicin on the 7th day. Blood sampling was done to measure the serum level of creatinine (creatinine) to evaluate the kidney and creatine kinase (CK) to evaluate the heart muscles along with the evaluation of total oxidant level (TOS) and total antioxidant capacity (TAC). Histopathological examinations were performed for both organs of the heart and kidneys. The lesions were graded from 0 to 9 based on the severity of the lesions.
Results: Doxorubicin administration resulted in significant cardiac and renal damage, evident by macroscopic and microscopic alterations, increased serum creatinine levels, and elevated creatine kinase concentrations. The 150 mg/kg AGE group demonstrated the most pronounced protective effects, reducing tissue damage and enhancing total antioxidant capacity compared to the doxorubicin-only group. Although Higher doses of AGE accompanied higher TAC, they did not confer additional protective benefits and may have compromised renal function.
Conclusion: Aged Garlic Extract effectively mitigates doxorubicin-induced toxicity in cardiac and renal tissues, with a notable dose-dependent response. This research suggests that AGE might be a useful addition to doxorubicin treatment protocols, warranting further investigation into its mechanisms and potential clinical applications.
Type of Study: Original |
Subject:
Histology
Received: 2025/01/27 | Accepted: 2025/02/16 | Published: 2023/08/30
Received: 2025/01/27 | Accepted: 2025/02/16 | Published: 2023/08/30
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