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ASJ 2004, 2(1): 37-45 |
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Imani H, Hasani F, Haeri Rouhani S A, Nasr Esfahani M H, Dalman A, Reza Zadeh M, et al . Effect of Beta-Mercaptoethanol on in Vitro Maturation and Development of Immature Mouse Oocytes. ASJ 2004; 2 (1) :37-45
URL: http://anatomyjournal.ir/article-1-361-en.html
URL: http://anatomyjournal.ir/article-1-361-en.html
Hosein Imani 
1, Fatemeh Hasani , Seyyed Ali Haeri Rouhani , Mohammad Hosein Nasr Esfahani , Azam Dalman , Mojtaba Reza Zadeh , Saeed Kazemi Ashtiani , Abdolhosein Shahverdi , Hosein Bahar Vand
1, Fatemeh Hasani , Seyyed Ali Haeri Rouhani , Mohammad Hosein Nasr Esfahani , Azam Dalman , Mojtaba Reza Zadeh , Saeed Kazemi Ashtiani , Abdolhosein Shahverdi , Hosein Bahar Vand
1- Embryology Department, Royan Institute, Tehran, Iran.
Abstract: (858 Views)
Purpose: This Study evaluated the resumption of meiosis, in vitro maturtion and department of immature mouse oocytes with b- mercaptothanol.
Materials and methods: Immature oocytes were recovered from NMRI mouse strain (4-6 weeks) in different 4 experiments groups: the control group, the oocytes were matured in MEM-a+ medium containing + FCS 55%; group 1: the oocytes were matured in MEM- a medium containing + FCS 5% and b-mercaptoethanol; group 2: the oocytes were matured in MEM-a+ FCS 5%, rFSH 100mIU and group 3: the oocytes were mature in MEM- a medium containing, FCS 5%, hCG 7.5IU, rFSH100mIU, b-mer-captoethanol 100 mm. Fertilization and embryo development were done in T6 medium.
Results: There were significant differences on in vitro maturation between control group than the group 1 and group 3 (p=0.01, p=0.007) and resumption of miosis between control group than the group 1 and group 3 (p=0.009, p=0.001).The rates of the resumpsion of meiosis in experimental groups were 74.20 (control), 82.71 (group 1), 84.49 (group 2) and 85.49% (group 3), In vitro maturation also increased significantly with (3-mercapthoethanol (group 1=69.49%, group 3=70.22%).The embryo formation on the fourth day in group 1 than the control group, group 2, group 3 (p=0.001, p=0.000,p=0.000) increased more significantly than that in the other groups.
Conclusion: The results of this study showed that b-mer-captoethanol (100 mm)has an effect on in vitro maturation and resumption of meiosis of mouse oocytes and improves embryo development.
Materials and methods: Immature oocytes were recovered from NMRI mouse strain (4-6 weeks) in different 4 experiments groups: the control group, the oocytes were matured in MEM-a+ medium containing + FCS 55%; group 1: the oocytes were matured in MEM- a medium containing + FCS 5% and b-mercaptoethanol; group 2: the oocytes were matured in MEM-a+ FCS 5%, rFSH 100mIU and group 3: the oocytes were mature in MEM- a medium containing, FCS 5%, hCG 7.5IU, rFSH100mIU, b-mer-captoethanol 100 mm. Fertilization and embryo development were done in T6 medium.
Results: There were significant differences on in vitro maturation between control group than the group 1 and group 3 (p=0.01, p=0.007) and resumption of miosis between control group than the group 1 and group 3 (p=0.009, p=0.001).The rates of the resumpsion of meiosis in experimental groups were 74.20 (control), 82.71 (group 1), 84.49 (group 2) and 85.49% (group 3), In vitro maturation also increased significantly with (3-mercapthoethanol (group 1=69.49%, group 3=70.22%).The embryo formation on the fourth day in group 1 than the control group, group 2, group 3 (p=0.001, p=0.000,p=0.000) increased more significantly than that in the other groups.
Conclusion: The results of this study showed that b-mer-captoethanol (100 mm)has an effect on in vitro maturation and resumption of meiosis of mouse oocytes and improves embryo development.
Type of Study: Original |
Subject:
Morphometry
Received: 2021/12/25 | Accepted: 2004/04/22 | Published: 2004/04/22
Received: 2021/12/25 | Accepted: 2004/04/22 | Published: 2004/04/22
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