Volume 20, Issue 1 (Winter & Spring-In Press 2023)                   ASJ 2023, 20(1): 0-0 | Back to browse issues page

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Bahadoran H, Asghari R, Moghadami S, Miri A, Habibi Azarian S. Case Report: Positive Post-Mortem Identification of Skeletal Remains of a Person Using Radiography and DNA Analysis. ASJ 2023; 20 (1)
URL: http://anatomyjournal.ir/article-1-619-en.html
1- Department of Anatomy, School of Medicine, Baqiyatallah Medical Science University, Tehran, Iran.
2- Human Genetic Research Center, Baqiyatallah University of medical sciences, Tehran, Iran
Abstract:   (106 Views)
Introduction: This study has surveyed the results obtained in identifying a missing person from Iran - Iraq War after 40 years in the Majnoon Island. A skeletal remains were examined by anthropometry. Parts of selected samples of femur and tooth were sampled for genetic identification.
DNA extraction from bone sample was performed using the QIAamp DNA Blood Mini Kit with a slight change. DNA was extracted using the Qiagen column, washing buffer, and bind-elute technology. Extracted DNA was quantitated by Quantifiler™ Trio DNA Quantification. According to DNA concentration, AmpFℓSTR Miniifiler PCR Amplification or AmpFℓSTR Identifiler PCR Amplification kits were used for genotyping. FTA cards were used to store and extract DNA samples from family. Genetic profiles were prepared using the AmpFℓSTR Identifiler Direct PCR Amplification Kit. The genetic matching of sex chromosomes was investigated with Yfiler™ PCR Amplification and Investigator Argus X-12 QS kits. Genetic matching and kinship calculations were done by software Familias and NoorGIS.
Result: Radiographic and genetic examinations ultimately confirmed that the dead body matched the relevant family. Despite the challenges with DNA-degraded samples, a good genetic profile was obtained for genetic identification.
Conclusion: Anthropometric (especially evidence of post mortem, for example, radiography) and genetic examinations ultimately confirmed that the dead body matched the relevant family.

 
     
Type of Study: Original | Subject: Morphometry
Received: 2024/04/27 | Accepted: 2024/06/8 | Published: 2024/01/20

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