Volume 20, Issue 1 (Winter & Spring-In Press 2023)                   ASJ 2023, 20(1): 0-0 | Back to browse issues page

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Ghaleh H E G, Sabour E S, Nadoushan M M, Jalali Kondori B. Histopathological evaluation of Pistacia atlantica extract effects on experimental model of diabetic wound. ASJ 2023; 20 (1)
URL: http://anatomyjournal.ir/article-1-618-en.html
1- Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
2- Department of Pharmacognosy and Traditional Pharmacy, Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran.
3- Department of Anatomical Sciences, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract:   (61 Views)
Introduction: Type 2 diabetes is the most common type of diabetes characterized by insulin resistance and beta cell dysfunction. The speed of wound healing is a very important issue in wound healing process. The conducted research shows that the use of natural products and traditional medicine methods in the treatment of many diseases and wounds has spread in the world. Therefore, the aim of the present study is to investigate the histopathological effectiveness of Pistacia atlantica extract (Beneh) in improving experimental model of diabetic wound. Methods: This study was conducted on 48 Balb/c mice. In order to induce type 2 diabetes, the animals were fed with a high-fat diet for 2 weeks, and then a single dose of 30 mg/kg was injected intraperitoneally. In this study, the wound was created by excisional wound splinting model and biopsy punch. Glucose level was measured with a glucometer and insulin level was measured with an ELISA kit. Histopathological examination was also done with hematoxylin/eosin staining and Masson trichrome staining.
Result: The results of the macroscopic study showed that the wound size was reduced in both the Pistacia atlantica extract and silver sulfadiazine groups compared to the wound control group. The results of hematoxylin and eosin staining also showed that In the treatment groups, a reduction in inflammation was observed in the wound area. The re-epithelialization occurred well in both treatment groups. However, its speed was higher in the Pistacia atlantica treated group than in the silver sulfadiazine group. Masson's trichrome staining results showed the collagen fibers in the Pistacia atlantica group have a more regular arrangement than silver and wound control groups.The results of the serum analysis also showed that the gum extract of the Pistacia atlantica reduced the production of NO and MPO in treatment groups compared to the control group.
Conclusion: The results of our study showed that the use of Pistacia atlantica extract topically in the diabetic wound area can improve the rate of closure and re-epithelialization in the diabetic wound  by reducing inflammation.
Type of Study: Original | Subject: Histology
Received: 2024/04/27 | Accepted: 2024/05/6 | Published: 2024/01/20

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