In Vitro Development of Cattle Embryo as Affected By Glucose, Serum and EDTA

Pirestani, Akbar; Hosseini,  Seyed Morteza; Moulavi , Fariba; Hajian , Mahdi; Abedi,  Parvaneh; Forouzanfar,  Mohsen; Ostad Hosseini, Somayeh; Hosseini,  Laleh; Ghasemzadeh Nava, Hamid; Tajik,  Parviz; Nasr Esfahani,  Mohammad Hasan

Volume 7, Issue 27 (Summer 2009) ASJ 2009, 7(27): 11-23 | Back to browse issues page

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Pirestani A, Hosseini S M, Moulavi F, Hajian M, Abedi P, Forouzanfar M, et al . In Vitro Development of Cattle Embryo as Affected By Glucose, Serum and EDTA. ASJ 2009; 7 (27) :11-23
URL: http://anatomyjournal.ir/article-1-485-en.html

In Vitro Development of Cattle Embryo as Affected By Glucose, Serum and EDTA

Akbar Pirestani

, Seyed Morteza Hosseini

, Somayeh Ostad Hosseini

, Laleh Hosseini

, Hamid Ghasemzadeh Nava

, Parviz Tajik

, Mohammad Hasan Nasr Esfahani

Abstract: (722 Views)

Purpose: The aim of this study was to evaluate the effect of different modifications of sequential synthetic oviductal_fluid (SOF) culture system on developmental competence of in vitro matured/fertilized cattle embryos. Materials and Methods: Bovine oocytes were matured and fertilized in vitro and then presumptive zygotes were randomly cultured for up to 9 days in different modifications of SOF culture system to consider the effects of glucose, serum and EDTA on embryo development. Results: All the embryo culture systems were efficient to support bovine embryo development till blastocyst stage. There was no significant difference in the ratios of embryos; however, the ratios of blastocyst and also hatchability of embryos cultured in SOF C (51.3%, 43.0% and 83.8%, respectively) were significantly higher than those of all the other SOF groups. Furthermore, while glucose had a partial improving effect on embryo development, a significant decrease in embryo development beyond the morula stage was observed in embryos cultured in SOF system with initial supplementation of EDTA compared with all the other groups. Conclusion: It was concluded that appropriate modifications of SOF culture systems can result insignificantly great in vitro embryo development.

Keywords: In Vitro Embryo Development, Bovine Zygotes, Sof, Serum

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Type of Study: Original | Subject: Morphometry
Received: 2021/12/26 | Accepted: 2009/07/19 | Published: 2009/07/19

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