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Volume 5, Issue 19 And 20 (Summer 2007)
ASJ 2007, 5(19 And 20): 157-166 |
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Sadri S, Davari Zanjani M, Azadbakht M, Amini A, Amiri I. Effect of Different Doses of Staurosporine For Neuronal Differentiation. ASJ 2007; 5 (19 and 20) :157-166
URL: http://anatomyjournal.ir/article-1-483-en.html
URL: http://anatomyjournal.ir/article-1-483-en.html
1- Biology Department, Faculty Of Sciences, Razi University, Kermanshah, Iran.
Abstract: (924 Views)
Purpose: The present study was designed to investigate the effect of different doses of Staurosporine on differentiation of PC-12 cell line into differentiated neuronal cells.
Materials and Methods: PC-12 cell line as a useful model system for neurobiological studies was maintained in RPMI 1640 supplemented with 10% FBS. Staurosporine were added to culture medium in different concentrations (110, 214, 316 and 1000nM), then morphology and apoptotic index were assessed by total neurite length estimation and TUNEL staining.
Results: Data showed that total neurite length of PC-12 cells that were treated with 110, 214, 316 and 1000nM was 44±9.3, 153±15.0, 104±10.4 and 31±9.6μm respectively. Therefore PC-12 cells that were treated with 214nM Staurosporine had the greatest neurite length in compression with the other concentrations (P<0.05). In addition apoptotic index in these concentrations was 3±0.7,4±0.8,8±1.0 and 10±1.1 respectively, Therefore PC-12 cells that were treated with 110 and 214nM Staurosporine had minimal apoptotic index (P<0.05).
Conclusion: Finally it can be concluded that 214nM concentration of Staurosporine is an optimum concentration for differentiation of neuronal cell lines to differentiated neuronal cells.
Materials and Methods: PC-12 cell line as a useful model system for neurobiological studies was maintained in RPMI 1640 supplemented with 10% FBS. Staurosporine were added to culture medium in different concentrations (110, 214, 316 and 1000nM), then morphology and apoptotic index were assessed by total neurite length estimation and TUNEL staining.
Results: Data showed that total neurite length of PC-12 cells that were treated with 110, 214, 316 and 1000nM was 44±9.3, 153±15.0, 104±10.4 and 31±9.6μm respectively. Therefore PC-12 cells that were treated with 214nM Staurosporine had the greatest neurite length in compression with the other concentrations (P<0.05). In addition apoptotic index in these concentrations was 3±0.7,4±0.8,8±1.0 and 10±1.1 respectively, Therefore PC-12 cells that were treated with 110 and 214nM Staurosporine had minimal apoptotic index (P<0.05).
Conclusion: Finally it can be concluded that 214nM concentration of Staurosporine is an optimum concentration for differentiation of neuronal cell lines to differentiated neuronal cells.
Type of Study: Original |
Subject:
Morphometry
Received: 2021/12/26 | Accepted: 2007/07/28 | Published: 2007/07/28
Received: 2021/12/26 | Accepted: 2007/07/28 | Published: 2007/07/28
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