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Volume 3, Issue 3 (Autumn 2005)
ASJ 2005, 3(3): 185-190 |
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Naderian H, Kazemi B, Hoseini A, Sadeghi Y, De Vries A. Molecular Cloning and Mutagenesis of Rat Glucocerebrosidase Gene. ASJ 2005; 3 (3) :185-190
URL: http://anatomyjournal.ir/article-1-414-en.html
URL: http://anatomyjournal.ir/article-1-414-en.html
1- Anatomy Department, Medical Facalty, Kashan Medical Sciences University, Kashan, Iran.
Abstract: (1109 Views)
Purpose: The aim of this study was cloning the Gba enzyme in pUCBM21 plasmid, and making frame mutation on it and sequencing it.
Materials and methods: mRNA was extracted from mouse spleen and glucocerebrosidase cDNA was synthesized and amplified by PCR with specific primers. cDNA was cloned in pUCBM21 and analyzed by restriction enzymes. A fragment of its sequence was deleted using MscI restriction enzyme .Then frame mutation was prepared and sequenced.
Results: glucocerebrosidase cDNA was synthesized and amplified by PCR. Then cloned in pUCBM21 and confirmed using restriction analysis. An in frame mutation was done and confirmed by sequencing.
Conclusion: The successful sequenced Gba cDNA compared with other researchers results.
Materials and methods: mRNA was extracted from mouse spleen and glucocerebrosidase cDNA was synthesized and amplified by PCR with specific primers. cDNA was cloned in pUCBM21 and analyzed by restriction enzymes. A fragment of its sequence was deleted using MscI restriction enzyme .Then frame mutation was prepared and sequenced.
Results: glucocerebrosidase cDNA was synthesized and amplified by PCR. Then cloned in pUCBM21 and confirmed using restriction analysis. An in frame mutation was done and confirmed by sequencing.
Conclusion: The successful sequenced Gba cDNA compared with other researchers results.
Type of Study: Original |
Subject:
Morphometry
Received: 2021/12/26 | Accepted: 2005/10/18 | Published: 2005/10/18
Received: 2021/12/26 | Accepted: 2005/10/18 | Published: 2005/10/18
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