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Volume 2, Issue 4 (Winter 2005)
ASJ 2005, 2(4): 33-39 |
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Hatami L, Reza Zadeh M. Differentiation of P19 Carcinoma Cell Line into Cardiomyocytes by Oxytocin Hormone. ASJ 2005; 2 (4) :33-39
URL: http://anatomyjournal.ir/article-1-392-en.html
URL: http://anatomyjournal.ir/article-1-392-en.html
1- Anatomy Department, Medical Sciences Faculty, Tarbiat Modarres University, Tehran, Iran.
Abstract: (1070 Views)
Purpose: The Present study was designed to investigate the OT effects on differentiation of P19 carcinoma cell line into cardiomyocytes.
Materials and Methods: P19 carcinoma cell line were cultivated in hanging drops for 2 days to form aggregates termed embryoid bodies (EBs) and in suspension for 5 days. The EBs was treated with oxytocin hormone and DMSO. The EBs were then plated onto gelatin- coated tissue culture dishes, and monitored microscopically daily for the presence of contracting areas, as well as the distribution of the timing of onset of spontaneous beating and beating frequency per minute was evaluated for each EBs. Cells from the spontaneously contracting areas within EBs were stained with anti a- actinin, anti-desmin, and anti-cardiac troponin I in 21- day- old EBS.
Results: Spontaneously contracting areas appeared in most of the EBs of OT and DMSO groups in 7+3 and 7+5 day, respectively. Immunohistological analysis showed that the harvested cardiomyocytes were stained positively with all of and bodies and displayed many features characteristic of native cardiomyocytes.
Conclusion: These results indicate that oxytocin hormone can induce differentiation of P19 carcinoma cells to cardimoyocytes, but the exact mechanism of this process is unclear. Thus, the OT-OTR system can play an important role in cardiogenesis even in vitro.
Materials and Methods: P19 carcinoma cell line were cultivated in hanging drops for 2 days to form aggregates termed embryoid bodies (EBs) and in suspension for 5 days. The EBs was treated with oxytocin hormone and DMSO. The EBs were then plated onto gelatin- coated tissue culture dishes, and monitored microscopically daily for the presence of contracting areas, as well as the distribution of the timing of onset of spontaneous beating and beating frequency per minute was evaluated for each EBs. Cells from the spontaneously contracting areas within EBs were stained with anti a- actinin, anti-desmin, and anti-cardiac troponin I in 21- day- old EBS.
Results: Spontaneously contracting areas appeared in most of the EBs of OT and DMSO groups in 7+3 and 7+5 day, respectively. Immunohistological analysis showed that the harvested cardiomyocytes were stained positively with all of and bodies and displayed many features characteristic of native cardiomyocytes.
Conclusion: These results indicate that oxytocin hormone can induce differentiation of P19 carcinoma cells to cardimoyocytes, but the exact mechanism of this process is unclear. Thus, the OT-OTR system can play an important role in cardiogenesis even in vitro.
Type of Study: Original |
Subject:
Morphometry
Received: 2021/12/26 | Accepted: 2004/01/20 | Published: 2004/01/20
Received: 2021/12/26 | Accepted: 2004/01/20 | Published: 2004/01/20
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