Sat, May 10, 2025
Volume 5, Issue 21 And 22 (Winter & Spring 2007)
ASJ 2007, 5(21 And 22): 309-309 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Baharvand H, Pakzad M, Taee A, Moula Mohammadi S, Rezaei M. Establishment, Culture and Freezing of Human and Mouse Embryonic Stem Cells: a Protocol Guide. ASJ 2007; 5 (21 and 22) :309-309
URL: http://anatomyjournal.ir/article-1-362-en.html
URL: http://anatomyjournal.ir/article-1-362-en.html
Abstract: (1092 Views)
|
Studies of the biology of human embryonic stem cells (hES cells) have developed rapidly over the past nine years since the first reports of their derivation. They clearly offer enormous potential, not only for regenerative medicine, but also for drug discovery and toxicology, human developmental biology and cancer research. Realizing these potentials a better understanding of the fundamental aspects of hES cells in vitro including maintaining , the quality of cells in their pluripotent state as well as improving aspects of experimental preparations , subpassaging and cryopereservation .The basic protocol describes the generation of mouse ES cells on mouse embryonic fibroblasts to provide simple method to hES cell establishment . Here we describe step by step the protocol of mouse and human ES cell generation, maintenance, subculturing , and cry preservation.
|
|
Type of Study: Original |
Subject:
Morphometry
Received: 2021/12/25 | Accepted: 2008/12/30 | Published: 2008/12/30
Received: 2021/12/25 | Accepted: 2008/12/30 | Published: 2008/12/30
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright © The Author(s);
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-By-NC), which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Contact Information
