Volume 16, Issue 1 (Winter & Spring 2019)                   ASJ 2019, 16(1): 13-22 | Back to browse issues page

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Barmaki B, Ghaffari H, Gholami S, Alipoor Tabrizi M. Effects of Aloe Vera Gel Extract on Aging-Associated Histological Changes of the Sciatic Nerve. ASJ 2019; 16 (1) :13-22
URL: http://anatomyjournal.ir/article-1-209-en.html
1- Department of Physiology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran.
2- Department of Anatomical Sciences, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran.
3- Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
4- Department of Heart, Emam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract:   (6822 Views)
Introduction: Prior qualitative and quantitative studies have reported morphological changes that occur in nerve fibers and non-neuronal cells of peripheral nerve during the lifetime of the rats. Previous studies suggest that Aloe Vera has some beneficial and therapeutic effects such as anti-oxidant effects. In the current study, we have evaluated histological and histomorphometric changes of Sciatic nerve in aged rats treated with Aloe Vera gel extract.
Methods: Sixty healthy male albino Sprague-Dawley rats weighing 200-250 g were randomly divided into two groups: control (normal diet), and experimental group (normal diet+Aloe Vera extract). Rats of the experimental group received 400 mg/kg Aloe Vera extract daily. Within 16, 20 and 24 months, animals were anesthetized with sodium thiopental (40 mg/kg) via IP injection. After removal of Sciatic nerve on the left side, nerve dissected and fixed in 4% glutaraldehyde, post fixed in osmium tetroxide 1%, dehydrated and then embedded in TAAB resin. Thin sections (1 μm) were stained with toluidine blue stain and ten slides were obtained from each animal and got examined on light microscope. Masson’s trichrome stain was used to evaluate the development of fibrosis. Histomorphometric and histologic criteria on Sciatic nerve were determined and data were recorded. Data were analyzed using SPSS software. 
Results: Results of the current study confirmed accumulation of collagen in the perineurium by aging. Our data suggested that aging caused a decrease in diameter of nerve trunk and nerve fibers, thickness of myelin and nerve fiber area. The nerve fascicle diameter decreased in the control group, only. The numbers of myelinated fibers with enfolding into the axoplasm and out folding, irregularity of nerve fibers, myelin sheath with unclear boundaries and alteration in myelin compaction were also increased. Treatment with Aloe Vera gel prevented nerve trunk, as well as fiber and myelin thickness changes.
Conclusion: In the current study, we observed some alterations in sciatic nerve caused by aging, such as accumulation of collagen in perineurieum and endoneurieum. Also, a significant decrease was seen in nerve fibers,nerve trunks diameter and myelin thickness which were highly evident in rats of the 24 months age group. Treatment with Aloe vera gel extract improved histological changes and retarded neuropathy signs, significantly.
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Type of Study: Original | Subject: Histology
Received: 2018/02/13 | Accepted: 2018/07/5 | Published: 2019/01/1

1. Verdú E, Ceballos D, Vilches JJ, Navarro X. Influence of aging on peripheral nerve function and regeneration. Journal of the Peripheral Nervous System. 2000; 5(4):191-208. [DOI:10.1046/j.1529-8027.2000.00026.x] [PMID] [DOI:10.1046/j.1529-8027.2000.00026.x]
2. Mold JW, Vesely SK, Keyl BA, Schenk JB, Roberts M. The prevalence, predictors, and consequences of peripheral sensory neuropathy in older patients. The Journal of the American Board of Family Practice. 2004; 17(5):309-18. [DOI:10.3122/jabfm.17.5.309] [PMID] [DOI:10.3122/jabfm.17.5.309]
3. Verghese J, Bieri P, Gellido C, Schaumburg H, Herskovitz S. Peripheral neuropathy in young-old and old-old patients. Muscle & Nerve. 2001; 24(11):1476-81. [DOI:10.1002/mus.1171] [PMID] [DOI:10.1002/mus.1171]
4. Hashizume K, Kanda K. Differential effects of aging on motoneurons and peripheral nerves innervating the hindlimb and forelimb muscles of rats. Neuroscience Research. 1995; 22(2):189-96. [DOI:10.1016/0168-0102(95)00889-3] [DOI:10.1016/0168-0102(95)00889-3]
5. Majeed S. Survey on spontaneous peripheral neuropathy in aging rats. Arzneimittel-Forschung. 1992; 42(7):986-90. [PMID] [PMID]
6. Melcangi R, Magnaghi V, Cavarretta I, Martini L, Piva F. Age-induced decrease of glycoprotein Po and myelin basic protein gene expression in the rat sciatic nerve: Repair by steroid derivatives. Neuroscience. 1998; 85(2):569-78. [DOI:10.1016/S0306-4522(97)00628-3] [DOI:10.1016/S0306-4522(97)00628-3]
7. Knox CA, Kokmen E, Dyck PJ. Morphometric alteration of rat myelinated fibers with aging. Journal of Neuropathology & Experimental Neurology. 1989; 48(2):119-39. [DOI:10.1097/00005072-198903000-00001] [DOI:10.1097/00005072-198903000-00001]
8. Verdú E, Butí M, Navarro X. Functional changes of the peripheral nervous system with aging in the mouse. Neurobiology of Aging. 1996; 17(1):73-7. [DOI:10.1016/0197-4580(95)02010-1] [DOI:10.1016/0197-4580(95)02010-1]
9. Coleman P, Finch C, Joseph J. The need for multiple time points in aging studies. Neurobiology of Aging. 1990; 11(1):1-2. [DOI:10.1016/j.neurobiolaging.2003.10.002] [DOI:10.1016/j.neurobiolaging.2003.10.002]
10. Thomas P, King R, Sharma A. Changes with age in the peripheral nerves of the rat. Acta Neuropathologica. 1980; 52(1):1-6. [DOI:10.1007/BF00687222] [PMID] [DOI:10.1007/BF00687222]
11. Rajasekaran S, Ravi K, Sivagnanam K, Subramanian S. Beneficial effects of Aloe vera leaf gel extract on lipid profile status in rats with streptozotocin diabetes. Clinical and Experimental Pharmacology and Physiology. 2006; 33(3):232-7. [DOI:10.1111/j.1440-1681.2006.04351.x] [PMID] [DOI:10.1111/j.1440-1681.2006.04351.x]
12. Rajasekaran S, Sivagnanam K, Subramanian S. Antioxidant effect of Aloe vera gel extract in streptozotocin-induced diabetes in rats. Pharmacological Reports. 2005; 57(1):90-6. [PMID] [PMID]
13. Vogler B, Ernst E. Aloe vera: A systematic review of its clinical effectiveness. British Journal of General Practice. 1999; 49(447):823-8. [PMID] [PMCID] [PMID] [PMCID]
14. Sahu PK, Giri DD, Singh R, Pandey P, Gupta S, Shrivastava AK, et al. Therapeutic and medicinal uses of Aloe vera: A review. Pharmacology & Pharmacy. 2013; 4(08):599. [DOI:10.4236/pp.2013.48086] [DOI:10.4236/pp.2013.48086]
15. Bozzola JJ, Russell LD. Electron microscopy: Principles and techniques for biologists. Burlington, Massachusetts: Jones & Bartlett Learning; 1999.
16. Tseng H, Lin SE, Chang YL, Chen MH, Hung SH. Determining the critical effective temperature and heat dispersal pattern in monopolar radiofrequency ablation using temperature-time integration. Experimental and Therapeutic Medicine. 2016; 11(3):763-8. [DOI:10.3892/etm.2015.2956] [PMID] [PMCID] [DOI:10.3892/etm.2015.2956]
17. Jeronimo A, Jeronimo CAD, Sanada LS, Fazan VPS. Microscopic anatomy of the sural nerve in the postnatal developing rat: A longitudinal and lateral symmetry study. Journal of Anatomy. 2005; 206(1):93-9. [DOI:10.1111/j.0021-8782.2005.00368.x] [PMID] [PMCID] [DOI:10.1111/j.0021-8782.2005.00368.x]
18. Ceballos D, Cuadras J, Verdú E, Navarro X. Morphometric and ultrastructural changes with ageing in mouse peripheral nerve. Journal of Anatomy. 1999; 195(4):563-76. [DOI:10.1046/j.1469-7580.1999.19540563.x] [PMID] [PMCID] [DOI:10.1046/j.1469-7580.1999.19540563.x]
19. Krinke G, Froehlich E, Herrmann M, Schnider K, Da Suva F, Suter J, et al. Adjustment of the myelin sheath to axonal atrophy in the rat spinal root by the formation of infolded myelin loops. Cells Tissues Organs. 1988; 131(3):182-7. [DOI:10.1159/000146510] [DOI:10.1159/000146510]
20. Peters A. The effects of normal aging on myelin and nerve fibers: A review. Journal of Neurocytology. 2002; 31(8-9):581-93. [DOI:10.1023/A:1025731309829] [PMID] [DOI:10.1023/A:1025731309829]
21. Jeronimo A, Jeronimo CAD, Rodrigues Filho OA, Sanada LS, Fazan VPS. A morphometric study on the longitudinal and lateral symmetry of the sural nerve in mature and aging female rats. Brain Research. 2008; 1222:51-60. [DOI:10.1016/j.brainres.2008.05.055] [PMID] [DOI:10.1016/j.brainres.2008.05.055]
22. Schellens RL, van Veen BK, Gabreëls-Festen AA, Notermans SL, van't Hof MA, Stegeman DF. A statistical approach to fiber diameter distribution in human sural nerve. Muscle & Nerve. 1993; 16(12):1342-50. [DOI:10.1002/mus.880161212] [PMID] [DOI:10.1002/mus.880161212]
23. Berthold C, Carlstedt T, Corneliuson O. Anatomy of the nerve root at the central-peripheral transitional region. Peripheral Neuropathy. 1984; 1:156-70.
24. Romero E, Cuisenaire O, Denef J-F, Delbeke J, Macq B, Veraart C. Automatic morphometry of nerve histological sections. Journal of Neuroscience Methods. 2000; 97(2):111-22. [DOI:10.1016/S0165-0270(00)00167-9] [DOI:10.1016/S0165-0270(00)00167-9]
25. Bouche P, Cattelin F, Saint-Jean O, Leger J, Queslati S, Guez D, et al. Clinical and electrophysiological study of the peripheral nervous system in the elderly. Journal of Neurology. 1993; 240(5):263-8. [DOI:10.1007/BF00838158] [PMID] [DOI:10.1007/BF00838158]
26. Jacobs J, Love S. Qualitative and quantitative morphology of human sural nerve at different ages. Brain. 1985; 108(4):897-924. [DOI:10.1093/brain/108.4.897] [PMID] [DOI:10.1093/brain/108.4.897]
27. Esquisatto MAM, de Aro AA, Fêo HB, Gomes L. Changes in the connective tissue sheath of Wistar rat nerve with aging. Annals of Anatomy-Anatomischer Anzeiger. 2014; 196(6):441-8. [DOI:10.1016/j.aanat.2014.08.005] [PMID] [DOI:10.1016/j.aanat.2014.08.005]
28. Romanovsky D, Mrak RE, Dobretsov M. Age-dependent decline in density of human nerve and spinal ganglia neurons expressing the α3 isoform of Na/K-ATPase. Neuroscience. 2015; 310:342-53. [DOI:10.1016/j.neuroscience.2015.09.034] [PMID] [PMCID] [DOI:10.1016/j.neuroscience.2015.09.034]
29. Nagamatsu M, Schmelzer JD, Zollman PJ, Smithson IL, Nickander KK, Low PA. Ischemic reperfusion causes lipid peroxidation and fiber degeneration. Muscle & Nerve. 1996; 19(1):37-47. [DOI:10.1002/mus.880190103] [PMID] [DOI:10.1002/mus.880190103]
30. Ghaffari H, Gholami S, Naghdi M. Histologic and histomorphometric study of tibial nerve treated by hydro-alcoholic Aloe vera gel extract in diabetic male rats. Anatomical Sciences Journal. 2013; 10(2):63-72.
31. Saberi M, Gholami S. An investigation on the effects of the Aloe vera extract on the thickness of the retina in male diabetic rats. Iranian Journal of Veterinary Research. 2012; 13(4):296-302.
32. Guven M, Gölge UH, Aslan E, Sehitoglu MH, Aras AB, Akman T, et al. The effect of Aloe vera on ischemia—Reperfusion injury of sciatic nerve in rats. Biomedicine & Pharmacotherapy. 2016; 79:201-7. [DOI:10.1016/j.biopha.2016.02.023] [PMID] [DOI:10.1016/j.biopha.2016.02.023]
33. López Jornet P, Camacho Alonso F, Molino Pagan D. Prospective, randomized, double-blind, clinical evaluation of Aloe vera Barbadensis, applied in combination with a tongue protector to treat burning mouth syndrome. Journal of Oral Pathology & Medicine. 2013; 42(4):295-301. [DOI:10.1111/jop.12002] [PMID] [DOI:10.1111/jop.12002]

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