Wed, Nov 4, 2026
Volume 7, Issue 27 (Summer 2009)
ASJ 2009, 7(27): 25-31 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mohammadi Roushandeh A, Haji Hosseinlou H, Niknafs B, Halabian R, Rouhbakhsh M, Daneshvar M. Effects of Different Doses of Lif on Ivm Rate and Cumulus Expansion In Mouse Oocytes. , ASJ. 2009; 7 (27) :25-31
URL: http://anatomyjournal.ir/article-1-486-en.html
URL: http://anatomyjournal.ir/article-1-486-en.html
Ameneh Mohammadi Roushandeh *1 
, Hassan Haji Hosseinlou , Behrouz Niknafs , Raheleh Halabian , Mahdi Rouhbakhsh , Monireh Daneshvar
, Hassan Haji Hosseinlou , Behrouz Niknafs , Raheleh Halabian , Mahdi Rouhbakhsh , Monireh Daneshvar
Abstract: (2742 Views)
Purpose: The aim of this research was to study the effects of different doses of LIF on GVBD and MII development rate and cumulus expansion. Materials and Methods: Immature mice superovulated with HMG and GV oocytes obtained from ovary 48 hours later. The GV oocytes were cultured in TCM199 with 100, 500 and 1000 IU /ml LIF. Cumulus expansions were evaluated with two examiners and numbers of MII oocytes were recorded. For denuding the oocytes hyaloronidase was used.Results: Our results showed that the rate of GVBD and MII development increased in groups with LIF compared with control group. Rate of MII development with 1000 IU/ml LIF was significantly higherthan that of control group (P<0.05). Cumulus expansion in group with 1000 IU/ml LIF improved significantly compared with control group (p<0.05). Conclusion: Our results showed that LIF could improve IVM rate in dose dependant. Also cumulus expansion improved in group with LIF and increased oocyte quality.
Type of Study: Original |
Subject:
Morphometry
Received: 2021/12/26 | Accepted: 2009/07/19 | Published: 2009/07/19
Received: 2021/12/26 | Accepted: 2009/07/19 | Published: 2009/07/19
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright © The Author(s);
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-By-NC), which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Contact Information
