Volume 6, Issue 24 (Autumn 2008)                   ASJ 2008, 6(24): 447-457 | Back to browse issues page

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Khoub Bakht Z, Hajian M, Hosseini S M, Forouzanfar M, Moulavi F, Abedi P, et al . Effect of Demecolicne Treatment on The Developmental Competency of In Vitro Matured Bovine Oocytes. ASJ 2008; 6 (24) :447-457
URL: http://anatomyjournal.ir/article-1-368-en.html
Effect of Demecolicne Treatment on The Developmental Competency of In Vitro Matured Bovine Oocytes
Zeinab Khoub Bakht , Mahdi Hajian , Seyed Morteza Hosseini , Mohsen Forouzanfar , Fariba Moulavi , Parvaneh Abedi , Laleh Hosseini , Hamed Karami Shabankareh , Mohammad Hossein Nasresfahani 1
Abstract:   (833 Views)
Purpose: The aim of this study was to investigate whether demecolicne treatment of matured bovine oocytes adversely affects the process of in vitro fertilization and embryo development. Materials and Methods: Bovine Cumulus Oocyte Complexes (COC's) were matured in vitro and then were randomly allocated to two treatment groups of common concentrations of demecolicne (0.05 and 0.4 mg/ml for 30 min) and a control group. COC's were then fertilized and cultured in vitro for up to 9 days when the ratios of in vitro embryo development and the viability of the hatched blastocysts were assessed and compared with the control group (p<0.05). Results: The ratios of the cleavage and blastocyst formation of demecolicne treated groups (0.4 and 0.05 mg/ml) were 68.6, 63.5% and 23.3, 32.8%, which were not significantly different from the control group (73.3, 29.0%), respectively. The results of cell-viability were also not significantly different between the control vs. treatment groups. Conclusion: Since the overall indices of in vitro embryo development revealed no significant difference between the demecolicne treated compared to control bovine oocytes, it seems that demecolicne treatment of matured bovine oocytes may not compromise their potency for further in vitro development.
Keywords: Demecolicne, Bovine Oocyte, Developmental Competency, Viability
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Type of Study: Original | Subject: Morphometry
Received: 2021/12/25 | Accepted: 2008/10/19 | Published: 2008/10/19
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