Volume 12, Issue 4 (Autumn 2015)                   ASJ 2015, 12(4): 171-176 | Back to browse issues page

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Mir M, Arab M R, Shahraki M R, Mashhadi M A, Shahraki Salar M, Sargolzai Aval F et al . Toxic Effects of Cisplatin on Hepatocytes and Liver Enzymes of Rats. ASJ 2015; 12 (4) :171-176
URL: http://anatomyjournal.ir/article-1-130-en.html
1- Department of Anatomy, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
2- Department of Physiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
3- Department of Hematology and Oncology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
4- Department of Anatomy, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
Abstract:   (5713 Views)

Introduction: Cisplatin is an antineoplastic drug, which is widely used for the treatment of solid tumors. However, its clinical usage is limited because of its side effects such as hepatotoxicity. This study aimed to identify toxic effects of cisplatin on hepatocytes of rats.
Methods: A total of 45 adult Sprague Dawley rats weighing 200±30 g were randomly divided into experimental (n=30) and control (n=15) groups. Rats of experimental groups were divided into 2 subgroups; subgroup 1 received 2 consecutive 2.5mg/kg dose of cisplatin, intraperitoneally in the beginning of first and fifth weeks of the study. Subgroup 2 also received 2 consecutive 5mg/kg dose of cisplatin in the same manner of subgroup 1. After 8 weeks, rats of both groups were anesthetized and killed. Then, their blood and tissue samples were taken. Prepared sections were stained by HE method. Collected data from microscopic slides and blood samples were analyzed by SPSS using analysis of variance (ANOVA) and Tukey test.
Results: Statistical analysis showed significant differences in the activity of enzymes (ALT, AST, ALP) between control and experimental groups (P<0.001). Analysis of sinusoidal diameter also showed a significant difference between studied groups (P<0.001) too.
Conclusion: Cisplatin disorganizes the architecture of hepatic lobules and increases sinusoidal diameter in rat liver.

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Type of Study: Original |
Received: 2015/03/15 | Accepted: 2015/09/21 | Published: 2015/11/1

1. Cavaletti G, Fabbrica D, Minoia C, Frattola L, Tredici G. Carboplatin toxic effects on the peripheral nervous system of the rat. Annals of Oncology. 1998; 9(4):443-8. [DOI:10.1023/A:1008231925889] [PMID]
2. Jordan P, Carmo-Fonseca M. Molecular mechanisms involved in cisplatin cytotoxicity. Cellular and Molecular Life Sciences. 2000; 57(8-9):1229-35. [DOI:10.1007/PL00000762] [PMID]
3. Cavaletti G, Marmiroli P. Chemotherapy-induced peripheral neurotoxicity. Nature Reviews Neurology. 2010; 6(12):657-66. [DOI:10.1038/nrneurol.2010.160] [PMID]
4. Díaz R, Jordá MV, Reynes G, Aparicio J, Segura A, Amador R, Calderero V, Beltran A. Neoadjuvant cisplatin and etoposide, with or without tamoxifen, prior to radiotherapy in high-grade gliomas: A single-center experience. Anti-Cancer Drugs. 2005; 16(3):323-9. [DOI:10.1097/00001813-200503000-00012] [PMID]
5. Ezz-Din D, Gabry MS, Farrag AR, Abdel Moneim AE. Physiological and histological impact of Azadirachta indica (neem) leaves extract in a rat model of cisplatin-induced hepato and nephrotoxicity. Journal of Medical Plants Research. 2011; 5:5499-06.
6. Attyah AM, Ismail SH. Protective effect of ginger extract against cisplatin-induced hepatotoxicity and cardiotoxicity in rats. Iraqi Journal of Pharmaceutical Sciences. 2012; 21(1):27-33.
7. Zheng XN, Wang XW, Li LY, Xu ZW, Huang HY, Zhao JS, et al. Pu-erh tea powder preventive effects on cisplatin-induced liver oxidative damage in Wistar rats. Asian Pacific Journal of Cancer prevention. 2013; 15(17):7389-94. [DOI:10.7314/APJCP.2014.15.17.7389]
8. Antunes G, Darian D, Bianchi M. Protective effects of vitamin C against cisplatin-induced nephrotoxicity and lipid peroxidation in adult rats: a dose-dependant study. Pharmacological Research 2000; 41(4):405-11. [DOI:10.1006/phrs.1999.0600] [PMID]
9. Palipoch S, Punsawad C, Koomhin P, Suwannalert P. Hepatoprotective effect of curcumin and alpha-tocopherol against cisplatin-induced oxidative stress. BMC Complementary and Alternative Medicine. 2014; 14:111. doi: 10.1186/1472-6882-14-111 [DOI:10.1186/1472-6882-14-111]
10. Ko DW, Lee IC, Park SH, Moon C, Kong SS. Protective effect of pine bark extract against cisplatin-induced hepatotoxicity and oxidative stress in rats. Laboratory Animal Research. 2014; 30(4):174-180. [DOI:10.5625/lar.2014.30.4.174] [PMID] [PMCID]
11. Karadeniz A, Simsek N, Karakus E, Yildirim S, Kara A, Can I, et al. Royal jelly modulates oxidative stress and apoptosis in liver and kidneys of rats treated with cisplatin. Oxidative Medicine and Cellular Longevity. 2011; 2011:981793. doi: 10.1155/2011/981793. [DOI:10.1155/2011/981793]
12. Avci A, Çetin R, Ergüder İB, Devrim E, Kiliçoğlu B, Candir O, et al. Cisplatin causes oxidation in rat liver tissues: Possible protective effects of antioxidant food supplementation. Turkish Journal of Medical Sciences. 2008; 38(2):117-20.
13. El-Sayyad HI, Ismail MF, Shalaby FM, Abou-El-Magd RF, Gaur RL, Fernando A, et al. Histopathological effects of cisplatin, doxorubicin and 5-flurouracil (5-FU) on the liver of male albino rats. International Journal of Biological Sciences. 2009; 5(5):466-73. [DOI:10.7150/ijbs.5.466] [PMID] [PMCID]

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