Introduction: Cisplatin is an anti-cancer drug used in chemotherapy. One of the limiting side effects of cisplatin is decreasing genital gland function, azoospermia and oligospermia. Tribulus terrestris (TT) has been used as an aphrodisiac. The present study amid to investigate the protective effect of TT hydroalcoholic extract against cisplatin-induced apoptisis on testis in mice.

Methods: Male adult mice (n=30) were divided into control group and 4 experimental groups (n=6). Control group reicived saline, the first experimental group received cisplatin (5.5 mg/ kg) and other three experimental groups received cisplatin (5.5mg/kg) and different doses of hydroalcoholic extact of TT (100, 300 and 500 mg/kg/i.p) resepctively. Day after the last injection, histopathology and histomorphic analysis and also TUNEL assay on mice’s testis were performed. Weights of body and testis, seminiferous tubules diameter and apoptotic index were assessed. Data analysis was performed using one-way ANOVA followed by Turkeys’ test.

Results: The results showed that cisplatin leaded to a reduction in the weight of body and testes, and increased apoptotic index significantly compared to the control group (P<0.001), while in treated groups with TT, the weights of body and testis and seminiferous tubules diameter were significantly higher compared with cisplatin group (P<0.001), but apoptotic index did not show significant differences.

Conclusion: The study demonstrates that extract of TT could have protective effect on cisplatin- induced apoptosis of testis and seminiferous tubules diameter that may be related to the presence of antioxidant components acting via a multitude of central and peripheral mechanisms.

Introduction: Cisplatin is an antineoplastic drug, which is widely used for the treatment of solid tumors. However, its clinical usage is limited because of its side effects such as hepatotoxicity. This study aimed to identify toxic effects of cisplatin on hepatocytes of rats.
Methods: A total of 45 adult Sprague Dawley rats weighing 200±30 g were randomly divided into experimental (n=30) and control (n=15) groups. Rats of experimental groups were divided into 2 subgroups; subgroup 1 received 2 consecutive 2.5mg/kg dose of cisplatin, intraperitoneally in the beginning of first and fifth weeks of the study. Subgroup 2 also received 2 consecutive 5mg/kg dose of cisplatin in the same manner of subgroup 1. After 8 weeks, rats of both groups were anesthetized and killed. Then, their blood and tissue samples were taken. Prepared sections were stained by HE method. Collected data from microscopic slides and blood samples were analyzed by SPSS using analysis of variance (ANOVA) and Tukey test.
Results: Statistical analysis showed significant differences in the activity of enzymes (ALT, AST, ALP) between control and experimental groups (P<0.001). Analysis of sinusoidal diameter also showed a significant difference between studied groups (P<0.001) too.
Conclusion: Cisplatin disorganizes the architecture of hepatic lobules and increases sinusoidal diameter in rat liver.

Introduction: Cisplatin is a platinum based antineoplastic drug, which is widely used for treatment of solid tumors. The present investigation was carried out to study the nephrotoxic effects of double dose injection of cisplatin in rats, as an experimental model.
Methods: In this experimental study, 45 adult male Sprague Dawley rats with average weight of 200±30 g were randomly divided into two experimental (n=30) and one control (n=15) groups. Rats of experimental groups received two repeated doses of cisplatin intraperitoneally (2.5 mg/kg, experimental group E1 & 5 mg/kg, experimental group E2) in the beginning of first and fifth week of the experiment. Eight weeks after injection, rats of all groups were given deep anesthesia and killed. Blood samples were collected directly from their hearts for biochemical evaluation. Tissue samples were removed and prepared sections were stained with H&E, PAS, Masson trichrome, and PNA methods. Prepared microscopic slides were utilized for both histopathological and morphometrical studies. Collected data were analyzed by ANOVA and Tukey post hoc test using SPSS.
Results: Cisplatin administration induced a significant decrease in urinary space diameter of renal corpuscles in the experimental groups compared to the control group. This ultimately led to the urinary space obstruction in up to 95% of nephrons in experimental groups (P<0.001). There were significant difference between control and experimental groups (P<0.001) with regard to epithelial thickness of collecting duct, proximal and distal convoluted tubules. Moreover, there was a significant difference between control and experimental groups (P<0.001) with regard to the diameter of vasa recta. Acute tubular necrosis and urinary space obstruction were the main histological features of the experimental groups. There were also a significant elevation in serum level of BUN and creatinine in experimental group compared to those of control group.
Conclusion: Cisplatin induces acute tubular necrosis and urinary space obstruction and some other morphological changes in rat kidney, in a dose dependent manner.

Introduction: Cisplatin is a platinum-based drug widely used for the treatment of different cancers. Cell surface glycoconjugates play an important role in cell-cell interactions. The present investigation was carried out to study the toxic effects of double dose injection of cisplatin on cell surface glycoconjugates in rat as an experimental model.
Methods: In this experimental study, 45 adult male Sprague Dawley rats were used. Experimental group E1 and experimental group E2 received two repeated dose of 2.5 mg/kg and 5 mg/kg of cisplatin, respectively in the beginning of the first and fifth week of the experiment. After 8 weeks of injection, rats were killed. Tissue samples were removed and prepared sections were stained with H&E, PNA (Peanut agglutinin), and UEA (Ulex europaeus agglutinin) methods. Prepared microscopic slides were utilized for both histopathological and morphometrical studies. The obtained data were analyzed by ANOVA and Tukey tests using SPSS.
Results: Cisplatin administration induced a significant decrease in internal and external diameters of seminiferous tubules in the experimental groups compared to the control one (P<0.0001). There was also significant difference between control and experimental groups with regard to germinal epithelial thickness of seminiferous tubules (P<0.0001). Moreover, there was a significant difference between control and experimental groups with regard to spermatogenesis index (P<0.004). Also a significant elevation was seen in staining intensity of germinal epithelium to PNA and UEA in experimental groups, compared to control group (P<0.0001).
Conclusion: Cisplatin induces a dose-dependent morphological changes of germinal epithelium and extensive changes in distribution pattern of fucose- and Gal/GalNac-containing glycoconjugates in seminiferous epithelium in rats.