Volume 9, Issue 37 (Winter 2012)                   ASJ 2012, 9(37): 318-328 | Back to browse issues page

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Zhaleh H, Azadbakht M, Bidmeshkipour A, Haramshahi S M A, Kehtari M. Effects of Different Concentrations of Morphine on Staurosporine-Induced Neurite Outgrowth in Pc12 Cells. ASJ. 2012; 9 (37) :318-328
URL: http://anatomyjournal.ir/article-1-548-en.html
Abstract:   (254 Views)
Purpose: The present study was conducted to evaluate the effect of different concentrations of morphine on staurosporine-induced neurite outgrowth in PC12 cells. Materials and Methods: PC12 cells were cultured in RPMI1640 culture medium supplemented with 0.2% BSA. Cells were divided into three groups; Ι, ΙΙ and ΙΙΙ, culture in the presence of 50, 100 and 214 nM staurosporine respectively. In each group cells were treated with different concentrations (10-12, 10-10, 10-8, 10-6, 10-4 and 0.0 M) of morphine; treatments 1, 2, 3, 4, 5 and 6 respectively. The percentage of cytotoxicity was performed by lactate dehydrogenase assay. The percentage of death cell was assessed by Hoechst/PI differential staining. Total neurite length was measured.Results: In all groups, the percentage of cytotoxicity as well as the percentage of cell death in treatments 4 and 5 was higher and in treatments 1 and 2 was lower than that of treatment 6 (p<0.05). In group ΙΙΙ, total neurite length in treatments 1 and 2 was higher than that of treatment 6 and in group I, total neurite length in treatments 4 and 5 was lower than treatment 6 (p<0.05). Opioid receptor antagonist (naltrexone) was caused reversed the effects of morphine (p<0.05).Conclusion: Our results suggest that morphine at low concentrations should improve staurosporine-induced neurite outgrowth in PC12 cells.
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Type of Study: Original | Subject: Morphometry
Received: 2021/12/31 | Accepted: 2012/02/21 | Published: 2012/02/21

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