Volume 8, Issue 32 And 33 (Autumn & Winter 2010)                   ASJ 2010, 8(32 And 33): 190-196 | Back to browse issues page

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Kafilzadeh F, Karami Shabankareh H, Soltani L, Kazemine Jasemi V. Comparison of the Effects of Myo-Inosito Alone or in Combination with Mem Vitamins on Embryo Development in Sanjabi Sheep. ASJ 2010; 8 (32 and 33) :190-196
URL: http://anatomyjournal.ir/article-1-523-en.html
Abstract:   (546 Views)
Purpose: The objective of this study was to evaluate the effects of myo-inositol alone or in combination with MEM vitamins on embryo development in sanjabi sheep.Materials and Methods: Sheep Cumulus Oocytes Complexes (COCs) were matured in vitro at 39ºC, in humidified 5% CO2 atmosphere for 22-24 h. There were three treatments, culture in synthetic oviductal fluid medium (treatment I), culture in synthetic oviductal fluid medium supplemented with1×MEM vitamins (treatment II), culture in synthetic oviductal fluid medium supplemented with myo-inositol (treatment III), COCs were then fertilized and cultured in vitro for 8 days when the ratios of in vitro embryo development of the hatched blastocysts were assessed and compared with the control group.Results: The presence of myo-inpsitol significantly improved overall morula rates (34.39%) than that of control group (24.18%), but there was no difference between myo-inositol and 1×MEM vitamins in the percentage of embryos successfully developing to the morula stage (p<0.05). The addition of myo-inositol improved the mean blastocyst formation compared to the oocytes matured in medium containing no MEM (control) or 1×MEM vitamins (32.63, 13.96, and 21.06% respectively). However the mean percentage of cleavage rate was not substantialy different between treatments. Conclusion: These results suggest that adding myo-inositol to SOF medium be more beneficial for subsequent sheep embryonic development.
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Type of Study: Original | Subject: Morphometry
Received: 2021/12/30 | Accepted: 2010/11/7 | Published: 2010/11/7

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