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Volume 8, Issue 31 (Summer 2010)
ASJ 2010, 8(31): 117-127 |
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Hashemi Bani B, Razavi S, Esfandiari E, Karbasi S, Mardani M, Nadali F, et al . Designing of Human Cartilage Tissue, by Differentiation of Adipose-Derived Stem Cells With BMP-6 in Alginate Scaffold. ASJ 2010; 8 (31) :117-127
URL: http://anatomyjournal.ir/article-1-512-en.html
URL: http://anatomyjournal.ir/article-1-512-en.html
Batool Hashemi Bani 
, Shahnaz Razavi1 , Ebrahim Esfandiari , Saeid Karbasi , Mohammad Mardani , Fatemeh Nadali , Farzaneh Sadeghi , Fereshteh Haghighat , Shaghayegh Haghjou , Vahid Gouharian
, Shahnaz Razavi1 , Ebrahim Esfandiari , Saeid Karbasi , Mohammad Mardani , Fatemeh Nadali , Farzaneh Sadeghi , Fereshteh Haghighat , Shaghayegh Haghjou , Vahid Gouharian
Abstract: (1073 Views)
Purpose: In the present study the effect of BMP-6 was investigated on chondrogenesis of adiposederived stem cells. Materials and Methods: Mesenchymal stem cells derived from subcutaneous adipose tissue were cultured on alginate scaffold to induce chondrogenesis in experimental group, with chondrogenic medium having BMP-6 growth factor for 3 weeks. In control group medium without BMP-6 was applied. The harvested constructs were examined with immunohistochemical and RT-PCR methods for assessment of cartilage-specific characteristics. Results: The results of immunohistochemical method revealed the presence of typical cartilage extracellular matrix components such as type II collagen and aggrecan in constructs induced by BMP-6 growth factor on alginate scaffold. In addition evaluation of the results of RT-PCR analysis confirmed the expression of cartilage- specific genes, such as type II collagen and aggrecan, in the differentiated cells under the influence of growth factor BMP-6. Conclusion: It can be concluded that BMP-6 promotes chondrogenesis of ADSC in 3-D and adipose-derived stem cells could be used for cartilage tissue engineering.
Type of Study: Original |
Subject:
Morphometry
Received: 2021/12/27 | Accepted: 2010/08/19 | Published: 2010/08/19
Received: 2021/12/27 | Accepted: 2010/08/19 | Published: 2010/08/19
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