Volume 5, Issue 18 (Spring 2007)                   ASJ 2007, 5(18): 49-59 | Back to browse issues page

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Razavi S, Fathi F, Tavalaei M, Vahdati A, Nasr Esfahani M H. Relation Between Sperm Binding Ability to Hyaluronic Acid With Protamine Deficiency and DNAFragmentation. ASJ. 2007; 5 (18) :49-59
URL: http://anatomyjournal.ir/article-1-464-en.html
1- Royan Institute, Embryology Department, Tehran, Iran.
Abstract:   (240 Views)
Purpose: The best sperm selection by its binding ability to hyaluronic acid for ICSI and assessment of correlation between sperm binding ability to hyaluronic acid with sperm parameters, protamine deficiency and DNA fragmentation.
Materials and Methods: Semen samples collected from 55 infertile couples referring to ?? Fertility and Infertility center were collected during this study. Semen analysis was carried out according to WHO criteria. By incubation sperm to hyaluronic acid within microinjection dishes, can measure percentage of sperm binding to hyaluronic acid. Sperm morphology, protamine deficiency and DNA fragmentation were assessed by Papanicolaou staining, Chromomycin A3 (CMA3) staining, and SCD test, respectively.
Results: Percentage of sperm binding to hyaluronic acid has significant negative correlation with percentage of abnormal morphology (p=0.001) whereas a significant positive correlation was obtained with sperm concentration (p=0.001) and motility (p=0.001). Also, percentage of sperm binding to hyaluronic acid has significant negative correlation with protamine deficiency (p=0.028) and DNA fragmentation (p=0.035).
Conclusion: The results of present study suggest that sperm binding to hyaluronic acid show normal morphology and intact chromatin structure. Thus, we could select the best sperm for ICSI that binds to hyaluronic acid.
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Type of Study: Original | Subject: Morphometry
Received: 2021/12/26 | Accepted: 2007/04/1 | Published: 2007/04/1

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