Volume 14, Issue 2 (Spring 2017)                   ASJ 2017, 14(2): 55-62 | Back to browse issues page

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Motaghi S, Sadeghi M, Seyyedin S, Sepehri G, Kheirandish R. Histomorphometrical and Histopathological Evaluation of Hedera Helix Alcoholic Extract on Dermal Collagen Bundles. ASJ. 2017; 14 (2) :55-62
URL: http://anatomyjournal.ir/article-1-166-en.html
1- Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
2- Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.
3- Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Abstract:   (1254 Views)

Introduction: Hedera helix is an evergreen plant used in treatment of wounds in the Iranian folk medicine. One of the main stages in wound healing process is, the proliferative phase in which fibroblasts proliferate within the wounds and produce extracellular matrix. Collagen is the main constituent of extracellular matrix. So the aim of this study was to evaluate the effect of Hedera helix alcoholic extract on dermal collagen bundles in wound healing process.
Methods: 7 adult Oryctolagus Caniculus rabbits were enrolled in this experiment. Four wounds were created on each rabbit. Alcoholic extract of hedera helix dried leaves were prepared. Wounds were treated with eucerin, phenytoin, hedera helix 10% and 20% eucerin-based creams for 20 days. On day 21 (after the completion of the experiment), skin samples were taken and the collagen bundles in reticular and papillary layers of the dermis were evaluated histologically.
Results: Histological assessment of dermal collagen bundles in the reticular layer showed that both 10% and 20% hedera helix creams, increased collagen bundle thickness significantly, compared to eucerin treated wounds (P<0.001 and P<0.01). In papillary layer there was no significant effect in both groups. Compared to eucerin treated wounds, collagen bundles were more organized in phenytoin and hedera helix 10% and 20% cream treated wounds, in a Histomorphological point of view.
Conclusion: Our results showed that hedera helix contains phytochemicals which may act on the proliferative phase of wound healing process. 

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Type of Study: Original | Subject: Gross Anatomy
Received: 2016/11/10 | Accepted: 2017/02/20 | Published: 2017/05/1

1. Singer AJ, Dagum AB. Current management of acute cutaneous wounds. New England Journal of Medicine. 2008; 359(10):1037-46. [DOI:10.1056/NEJMra0707253] [PMID] [DOI:10.1056/NEJMra0707253]
2. Canty EG, Kadler KE. Procollagen trafficking, processing and fibrillogenesis. Journal of Cell Science. 2005; 118(7):1341-53. [DOI:10.1242/jcs.01731] [PMID] [DOI:10.1242/jcs.01731]
3. Caves JM, Kumar VA, Wen J, Cui W, Martinez A, Apkarian R, et al. Fibrillogenesis in continuously spun synthetic collagen fiber. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2010; 93(1):24-38. [PMID] [PMID]
4. Demidova-Rice TN, Hamblin MR, Herman IM. Acute and impaired wound healing: Pathophysiology and current methods for drug delivery, part 1: Normal and chronic wounds: Biology, causes, and approaaches to care. Advances in Skin & Wound Care. 2012; 25(7):349. [DOI:10.1097/01.ASW.0000416006.55218.d0] [PMID] [PMCID] [DOI:10.1097/01.ASW.0000416006.55218.d0]
5. Thakur R, Jain N, Pathak R, Sandhu SS. Practices in wound healing studies of plants. Evidence-Based Complementary and Alternative Medicine. 2011; 2011. [DOI:10.1093/ecam/neq012] [PMID] [PMCID] [DOI:10.1093/ecam/neq012]
6. Hamedi A, Zarshenas MM, Sohrabpour M, Zargaran A. Herbal medicinal oils in traditional Persian medicine. Pharmaceutical Biology. 2013; 51(9):1208-18. [DOI:10.3109/13880209.2013.777462] [PMID] [DOI:10.3109/13880209.2013.777462]
7. Lodhi S, Pawar RS, Jain AP, Singhai A. Wound healing potential of Tephrosia purpurea (Linn.) Pers. in rats. Journal of Ethnopharmacology. 2006; 108(2):204-10. [DOI:10.1016/j.jep.2006.05.011] [PMID] [DOI:10.1016/j.jep.2006.05.011]
8. Mandade RJ, Choudhuri A, Mashirkar V, Sakarkar D. Analgesic and antiinflammatory activities of hedera helix leaf extract. International Journal of Comprehensive Pharmacy. 2010; 1(4):1-3.
9. Holzinger F, Chenot JF. Systematic review of clinical trials assessing the effectiveness of ivy leaf (hedera helix) for acute upper respiratory tract infections. Evidence-Based Complementary and Alternative Medicine. 2011; 8(1):1-9. [DOI:10.1155/2011/382789] [PMID] [PMCID] [DOI:10.1155/2011/382789]
10. Lutsenko YU, Bylka WI, Matlawska I, Darmohray RO. Hedera helix as a medicinal plant. Herba Polonica. 2010; 56(1):83-96.
11. Sohrabi-Haghdost RV, Safarmashaei S. Comparison of in-vivo wound healing activity of Verbascum thapsus flower extract with zinc oxide on experimental wound model in rabbits. Advances in Environmental Biology. 2011; 5(7):1501-9.
12. Cross S, Naylor L, Coleman R, Teo T. An experimental model to investigate the dynamics of wound contraction. British Journal of Plastic Surgery. 1995; 48(4):189-97. [DOI:10.1016/0007-1226(95)90001-2] [DOI:10.1016/0007-1226(95)90001-2]
13. Nazem MN, Teymouri M, Jahantigh M. The histomorphometric and histopathologic effect of methionine on the epidermis and dermis layers of skin in rat. Comparative Clinical Pathology. 2016; 25(4):699-704. [DOI:10.1007/s00580-016-2250-0] [DOI:10.1007/s00580-016-2250-0]
14. Nazem MN, Tavakoli H, Sajjadian SM. Histomorphometrical and histopathological assessment of in ovo methionine injection on the skin layers and its collagen bundles of chicken embryo. Anatomical Sciences Journal. 2015; 12(3):115-20.
15. Tsala DE, Joseph MN, Heroyine LT, Solomon H. In vivo wound healing effect of water extract of stereospernum kunthianum stem-bark. Journal of Medicinal Herbs and Ethnomedicine. 2016; 2(1):1-6.
16. Samanta R, Pattnaik A, Pradhan K, Mehta B, Pattanayak S, Banerjee S. Wound healing activity of silibinin in mice. Pharmacognosy Research. 2016; 8(4):298-302. [DOI:10.4103/0974-8490.188880] [PMID] [PMCID] [DOI:10.4103/0974-8490.188880]
17. Nabiuni M, Oryan S, Ayyobipor M, Bagheri M. [Histochemical study of Verbascum speciocum extract's effects on the wound healing in rats (Persian)]. Journal of Cell & Tissue. 2011; 2(1):67-75.
18. Shetty S, Udupa S, Udupa L. Evaluation of antioxidant and wound healing effects of alcoholic and aqueous extract of Ocimum sanctum Linn in rats. Evidence-Based Complementary and Alternative Medicine. 2008; 5(1):95-101. [DOI:10.1093/ecam/nem004] [PMID] [PMCID] [DOI:10.1093/ecam/nem004]
19. Uddin G, Rauf A, Qaisar M, Latif A, Ali M. Preliminary phytochemical screening and antimicrobial activity of Hedera helix L. Middle-East Journal of Science Research. 2011; 8(1):198-202.
20. Amin ZA, Ali HM, Alshawsh MA, Darvish PH, Abdulla MA. Application of antrodia camphorata promotes rat's wound healing in vivo and facilitates fibroblast cell proliferation in-vitro. Evidence-Based Complementary and Alternative Medicine. 2015; 2015:1-14. [DOI:10.1155/2015/317693] [PMID] [PMCID] [DOI:10.1155/2015/317693]
21. Bueno FG, Panizzon GP, Mello EVSdL, Lechtenberg M, Petereit F, Mello JCPd, et al. Hydrolyzable tannins from hydroalcoholic extract from Poincianella pluviosa stem bark and its wound-healing properties: Phytochemical investigations and influence on in vitro cell physiology of human keratinocytes and dermal fibroblasts. Fitoterapia. 2014; 99(8):252-60. [DOI:10.1016/j.fitote.2014.10.007] [PMID] [DOI:10.1016/j.fitote.2014.10.007]
22. Priya KS, Arumugam G, Rathinam B, Wells A, Babu M. Celosia argentea Linn. leaf extract improves wound healing in a rat burn wound model. Wound Repair and Regeneration. 2004; 12(6):618-25. [DOI:10.1111/j.1067-1927.2004.12603.x] [PMID] [DOI:10.1111/j.1067-1927.2004.12603.x]
23. James O, Friday ET. Phytochemical composition, bioactivity and wound healing potential of Euphorbia heterophylla (Euphorbiaceae) leaf extract. International Journal on Pharmaceutical and Biomedical Research. 2010; 1(1):54-63.
24. Fikru A, Makonnen E, Eguale T, Debella A, Mekonnen GA. Evaluation of in vivo wound healing activity of methanol extract of Achyranthes aspera L. Journal of Ethnopharmacology. 2012; 143(2):469-74. [DOI:10.1016/j.jep.2012.06.049] [PMID] [DOI:10.1016/j.jep.2012.06.049]
25. Perumal Samy R, Chow VT. Pilot study with regard to the wound healing activity of protein from calotropis procera (Ait.) R. Br. Evidence-Based Complementary and Alternative Medicine. 2012; 2012:1-14. [DOI:10.1155/2012/294528] [PMID] [PMCID] [DOI:10.1155/2012/294528]
26. Ghosh PK, Gaba A. Phyto-extracts in wound healing. Journal of Pharmacy and Pharmceutical Science. 2013; 16(5):760-820. [DOI:10.18433/J3831V] [DOI:10.18433/J3831V]
27. Majumdar M, Nayeem N, Kamath JV, Asad M. Evaluation of Tectona grandis leaves for wound healing activity. Pakistan Journal of Pharmaceutical Sciences. 2007; 20(2):120-4. [PMID] [PMID]
28. Umadevi S, Mohanta G, Kalaichelvan V, Manavalan R. Studies on wound healing effect of Flaveria trinervia leaf in mice. Indian Journal of Pharmaceutical Sciences. 2006; 68(1):106-8. [DOI:10.4103/0250-474X.22979] [DOI:10.4103/0250-474X.22979]
29. Datta HS, Mitra SK, Patwardhan B. Wound healing activity of topical application forms based on ayurveda. Evidence-Based Complementary and Alternative Medicine. 2011; 8(1):1-10. [DOI:10.1093/ecam/nep015] [PMID] [PMCID] [DOI:10.1093/ecam/nep015]
30. Wojdyło A, Oszmiański J, Czemerys R. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chemistry. 2007; 105(3):940-9. [DOI:10.1016/j.foodchem.2007.04.038] [DOI:10.1016/j.foodchem.2007.04.038]
31. Jain S, Jain A, Jain S, Malviya N, Jain V, Kumar D. Estimation of total phenolic, tannins, and flavonoid contents and antioxidant activity of Cedrus deodara heart wood extracts. Egyptian Pharmaceutical Journal. 2015; 14(1):10-4. [DOI:10.4103/1687-4315.154690] [DOI:10.4103/1687-4315.154690]
32. Costa G, González-Manzano S, González-Paramás A, Figueiredo IV, Santos-Buelga C, Batista MT. Flavan hetero-dimers in the Cymbopogon citratus infusion tannin fraction and their contribution to the antioxidant activity. Food & Function. 2015; 6(3):932-7. [DOI:10.1039/C5FO00042D] [PMID] [DOI:10.1039/C5FO00042D]
33. Dudonné S, Vitrac X, Coutiere P, Woillez M, Mérillon JM. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. Journal of Agricultural and Food Chemistry. 2009; 57(5):1768-74. [DOI:10.1021/jf803011r] [PMID] [DOI:10.1021/jf803011r]
34. Ghasemzadeh A, Jaafar HZE, Rahmat A, Ashkani S. Secondary metabolites constituents and antioxidant, anticancer and antibacterial activities of Etlingera elatior (Jack) R.M.Sm grown in different locations of Malaysia. BMC Complementary and Alternative Medicine. 2015; 15(1):1-10. [DOI:10.1186/s12906-015-0838-6] [PMID] [PMCID] [DOI:10.1186/s12906-015-0838-6]
35. Gülçin İ, Mshvildadze V, Gepdiremen A, Elias R. Antioxidant activity of saponins isolated from ivy: α-hederin, hederasaponin-C, hederacolchiside-E and hederacolchiside-F. Planta Medica. 2004; 70(6):561-3. [DOI:10.1055/s-2004-827158] [PMID] [DOI:10.1055/s-2004-827158]

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