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Volume 4, Issue 1 (Spring 2006)
ASJ 2006, 4(1): 11-17 |
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Saki G, Sajadian F, Kalantar Mahdavi S R, Sobhani A G. The Effect of Different Concentration of Human Recombinant of Leukemia Inhibitory Factor on Sperms of Asthenospermic Men. ASJ 2006; 4 (1) :11-17
URL: http://anatomyjournal.ir/article-1-432-en.html
URL: http://anatomyjournal.ir/article-1-432-en.html
1- Anatomy Department, Jondi Shapour University, Ahvaz, Iran.
Abstract: (1022 Views)
Introduction: Leukemia inhibitory factor is a secreted glycoprotein with a range of molecular weight forms, from 38-67kd, resulting from differential glycosylation of protein of approximately 20Kd. LIF has the four a helix cytokine structure that contains six cysteine residues, already known to occur in many hematopoietic factor. LIF is expressed at high constitutive in human fallopian tubal epithelium. Some studies has been done on the efect of LIF on normal sperm motility and shown that this factor could improve normal sperm motility. Objective: In this project y knowing that there is no report avout the effect of LIF on low motility sperms of asthenospermic men we decide to determine effect of this factor on low motility sperms.
Materials and Methods: In this investigation the sperm sample of 15 infertile men who have already referred to IVF department of Imam Khomeini hospital were collected and then preparing them by successive stages for culturing in Ham's F10+FCS 10% medium that contained various concentrations (0 ng/ml, 3 ng/ml, 5 ng/ml, 10 ng/ml, 50 ng/ml) of LIF at 37oC and under 5% of Co2 in air for up to 48 hours. Sperm motion characteristics were measured using Makler chamber. Sperm survival was determined by hyposmotic swelling test. Collected data with ANOVA method by means of SPSS software were analyzed.
Results: Sperm Motility and survival were not significantly enhance until 6 hours exposure to different concentration of LIF but sperm progressive. Motility were significantly higher after 24h exposure to 10 ng/ml of LIF. P<0.05. The survival rate of sperm was significantly prolonged in that time exposure to 10 ng/ml of LIF P<0.05. progressive motility of sperms and survival rate were signiicantly higher after 48h exposure to 50 ng/ml of LIF. P<0.05.
Conclusion: It can be concluded that the effect of Leukemia Inhibitory Factor on sperm motility and survival were concentration dependent and LIF increased sperm motility and survival in vitro after at least 24 hours of hours of incubation and we could not find significantly differences in shorter incubation period.
Materials and Methods: In this investigation the sperm sample of 15 infertile men who have already referred to IVF department of Imam Khomeini hospital were collected and then preparing them by successive stages for culturing in Ham's F10+FCS 10% medium that contained various concentrations (0 ng/ml, 3 ng/ml, 5 ng/ml, 10 ng/ml, 50 ng/ml) of LIF at 37oC and under 5% of Co2 in air for up to 48 hours. Sperm motion characteristics were measured using Makler chamber. Sperm survival was determined by hyposmotic swelling test. Collected data with ANOVA method by means of SPSS software were analyzed.
Results: Sperm Motility and survival were not significantly enhance until 6 hours exposure to different concentration of LIF but sperm progressive. Motility were significantly higher after 24h exposure to 10 ng/ml of LIF. P<0.05. The survival rate of sperm was significantly prolonged in that time exposure to 10 ng/ml of LIF P<0.05. progressive motility of sperms and survival rate were signiicantly higher after 48h exposure to 50 ng/ml of LIF. P<0.05.
Conclusion: It can be concluded that the effect of Leukemia Inhibitory Factor on sperm motility and survival were concentration dependent and LIF increased sperm motility and survival in vitro after at least 24 hours of hours of incubation and we could not find significantly differences in shorter incubation period.
Type of Study: Original |
Subject:
Morphometry
Received: 2021/12/26 | Accepted: 2006/04/21 | Published: 2006/04/21
Received: 2021/12/26 | Accepted: 2006/04/21 | Published: 2006/04/21
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