Effect of Β-Mercaptoethanolon Resumption of Meiosis, in Vitro Maturation and Development of Immature Mouse Oocyte Treated With Dehp (Di-(2-Ethylhexy) Phathalae)

Imani, Hosein; Dalman, Azam; Sepehri, Hoori; Kazemi Ashtiani, Saeed; Hasani, Fatemeh; Reza Zadeh, Mojtaba; Shah Verdi, Abdol Hosein; Bahar Vand, Hosein

Volume 3, Issue 2 (Summer 2005) ASJ 2005, 3(2): 109-116 | Back to browse issues page

Mendeley

Zotero

RefWorks

Imani H, Dalman A, Sepehri H, Kazemi Ashtiani S, Hasani F, Reza Zadeh M, et al . Effect of Β-Mercaptoethanolon Resumption of Meiosis, in Vitro Maturation and Development of Immature Mouse Oocyte Treated With Dehp (Di-(2-Ethylhexy) Phathalae). ASJ 2005; 3 (2) :109-116
URL: http://anatomyjournal.ir/article-1-409-en.html

Effect of Β-Mercaptoethanolon Resumption of Meiosis, in Vitro Maturation and Development of Immature Mouse Oocyte Treated With Dehp (Di-(2-Ethylhexy) Phathalae)

Hosein Imani

¹, Azam Dalman

, Hoori Sepehri

, Saeed Kazemi Ashtiani

, Fatemeh Hasani

, Mojtaba Reza Zadeh

, Abdol Hosein Shah Verdi

, Hosein Bahar Vand

1- Embryology Department, Royan Institute, Tehran, Iran.

Abstract: (876 Views)

Purpose: The aim of this study was evaluated the effect of β -mercaptoethanol on resumption of meiosis, In vivo maturation of immature mouse oocytes treated with DEHP and embryo development.
Materials and Methods: NMRI mouse strain (4-6 weeks) were treated daily with 50,µI DEHP Group 1: 121 immature oocytes were matured in MEM-α medium contain FCS 5%, hCG 7.5 IU/ml, rFsH 100mIU/ml, Group 2: 120 immature oocytes were matured in MEMα medium contain FCS 5%, hCG 7.5IU/ml, rFsH 100mIU/ml and 100µm β-mercaptoethanol.Control group: 198 immature mouse oocytes recovered from nontreated mouse and were matured in MEMα medium contain FCS 5%, hCG 7.5IU/ml, rFSH 100mlU/ml. Fertilization and embryo development were done in T6 medium.
Results: The resumption of meiosis in experimental groups (1&2) were (71%, 79%) respectively. However the resumption of meiosis in experimental groups were significantly different compared to control group (89%) (p<0.05). The In vitro maturation in experimental groups (1,2) were (52%, 68%) respectively. However the in vitro maturation in one group was significantly different compared to control group (75%) (p=0.00) and second group was significantly higher than one experimental group (p<0.01).The embryo number, after 24th insemination in experimental groups (1&2) were (52%, 70%) respectively However the embryo number in first group was significantly lower than the control group (74%) (P<0.05) and second group was significantly higher than the first group (p<0.05).
Conclusion: The results of this study showed that the β-mercaptoethanol (100µM) inhibited the negative effect of DEHP on In vitro maturation and embryo development. The β-mercaptoethanol has improved the resumption of meiosis, In vito maturation and embryo development.

Keywords: Immature oocyte, In vitro maturation, Mouse, Β-mercaptoethanol, Dehp (Di-(2-ethylhexy) phathalae)

Type of Study: Original | Subject: Morphometry
Received: 2021/12/26 | Accepted: 2005/07/19 | Published: 2005/07/19

Rights and permissions
	This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb