Volume 16, Issue 1 (Winter & Spring 2019)                   ASJ 2019, 16(1): 31-38 | Back to browse issues page

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Azarbarz N, Bayat P, Jalali Mashayekhi F, Ghafarizadeh A A, Bayat M. Correlation Between Serum and Seminal Leptin and Nitric Oxide (Nitrite) in Asthenote-ratozoospermic Males. ASJ. 2019; 16 (1) :31-38
URL: http://anatomyjournal.ir/article-1-203-en.html
1- Students Research Committee, Arak University of Medical Sciences, Arak, Iran.
2- Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
3- Department of Biochemistry and Genetics, Arak University of Medical Sciences, Arak, Iran.
4- Infertility Center, Iranian Academic Center For Education Culture & Research (ACECR), Arak, Iran.
Abstract:   (2968 Views)
Introduction: Leptin is a critical metabolic signal linking nutrition and reproductive functions. Leptin receptors are expressed in germ cells, Sertoli and Leydig cells. Oxidative stress increases sperm DNA damages. Nitric oxide is a free radical, produced by most cells via Nitric Oxide (NO) synthase and plays stimulatory and inhibitory roles on cells. Physiological concentrations of NO are essential for the biology and physiology systems. However, a low level of NO has a detrimental effect on cells. This study aimed to determine the correlation between seminal and serum leptin and NO in asthenoteratozoospermic men.
Methods: Semen and blood samples were obtained from (n=52) males referring to the Arak Infertility Clinic, in Arak City, Iran. We examined each patient for serum hormones (LH, testosterone) by chemiluminescence. Serum and seminal leptin were measured by ELISA and seminal NO was measured by Griess reaction. 
Results: There were differences in serum leptin levels, and total testosterone, LH, and NO concentrations among the 2 groups. Asthenoteratozoospermic groups showed higher testosterone and LH levels than the normozoospermic group. Serum leptin levels of asthenoteratozoospermic men significantly increased in comparison with normozoospermic men. Seminal leptin levels of asthenoteratozoospermic men significantly decreased compared to normozoospermic men. NO levels in asthenoteratozoospermic men significantly decreased in comparison with normozoospermic cases. There were no significant correlations between serum and seminal leptin and seminal plasma NO.
Conclusion: Our results do not support the hypothesis that in vivo serum and seminal leptin affect NO synthesis. Also, our results revealed that nitrite in the seminal plasma is not able to entirely indicate in vivo NO synthesis.
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Type of Study: Original | Subject: Reproductive Biology
Received: 2018/10/15 | Accepted: 2018/07/9 | Published: 2019/01/1

1. Sharma R, Biedenharn KR, Fedor JM, Agarwal A. Lifestyle factors and reproductive health: Taking control of your fertility. Reproductive Biology and Endocrinology. 2013; 11:66. [DOI:10.1186/1477-7827-11-66] [PMID] [PMCID] [DOI:10.1186/1477-7827-11-66]
2. Alam N. Male factor infertility-basics revisited. Journal of the College of Physicians and Surgeons Pakistan. 2009; 19(4):205-6. [PMID] [PMID]
3. Curi S, Ariagno J, Chenlo P, Mendeluk G, Pugliese M, Sardi Segovia L, et al. Asthenozoospermia: Analysis of a large population. Archives of Andrology. 2003; 49(5):343-9. [DOI:10.1080/713828220] [PMID] [DOI:10.1080/713828220]
4. Homan G, Davies M, Norman R. The impact of lifestyle factors on reproductive performance in the general population and those undergoing infertility treatment: A review. Human Reproduction Update. 2007; 13(3):209-23. [DOI:10.1093/humupd/dml056] [PMID] [DOI:10.1093/humupd/dml056]
5. Fernandez CD, Bellentani FF, Fernandes GS, Perobelli JE, Favareto APA, Nascimento AF, et al. Diet-induced obesity in rats leads to a decrease in sperm motility. Reproductive Biology and Endocrinology. 2011; 9:32. [DOI:10.1186/1477-7827-9-32] [PMID] [PMCID] [DOI:10.1186/1477-7827-9-32]
6. Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM. Positional cloning of the mouse obese gene and its human homologue. Nature. 1994; 372(6505):425-32. [DOI:10.1038/372425a0] [PMID] [DOI:10.1038/372425a0]
7. Matson C, Wiater M, Weigle D. Leptin and the regulation of body adiposity. Diabetes Reviews. 1996; 4(4):488-508.
8. Bray GA, York DA. Leptin and clinical medicine: A new piece in the puzzle of obesity. The Journal of Clinical Endocrinology & Metabolism. 1997; 82(9):2771-6. [DOI:10.1210/jcem.82.9.4224] [PMID] [DOI:10.1210/jcem.82.9.4224]
9. Aquila S, Giordano F, Guido C, Rago V, Carpino A. Nitric oxide involvement in the acrosome reaction triggered by leptin in pig sperm. Reproductive Biology and Endocrinology. 2011; 9(1):133. [DOI:10.1186/1477-7827-9-133] [PMID] [PMCID] [DOI:10.1186/1477-7827-9-133]
10. Garcia-Galiano D, Allen SJ, Elias CF. Role of the adipocyte-derived hormone leptin in reproductive control. Hormone Molecular Biology and Clinical Investigation. 2014; 19(3):141-9. [DOI:10.1515/hmbci-2014-0017] [PMID] [PMCID] [DOI:10.1515/hmbci-2014-0017]
11. Abbasihormozi S, Shahverdi A, Kouhkan A, Cheraghi J, Akhlaghi AA, Kheimeh A. Relationship of leptin administration with production of reactive oxygen species, sperm DNA fragmentation, sperm parameters and hormone profile in the adult rat. Archives of Gynecology and Obstetrics. 2013; 287(6):1241-9. [DOI:10.1007/s00404-012-2675-x] [PMID] [DOI:10.1007/s00404-012-2675-x]
12. Ishikawa T, Fujioka H, Ishimura T, Takenaka A, Fujisawa M. Expression of leptin and leptin receptor in the testis of fertile and infertile patients. Andrologia. 2007; 39(1):22-7. [DOI:10.1111/j.1439-0272.2006.00754.x] [PMID] [DOI:10.1111/j.1439-0272.2006.00754.x]
13. El-Hefnawy T, Ioffe S, Dym M. Expression of the leptin receptor during germ cell development in the mouse testis. Endocrinology. 2000; 141(7):2624-30. [DOI:10.1210/endo.141.7.7542] [PMID] [DOI:10.1210/endo.141.7.7542]
14. Barash IA, Cheung CC, Weigle DS, Ren H, Kabigting EB, Kuijper JL, et al. Leptin is a metabolic signal to the reproductive system. Endocrinology. 1996; 137(7):3144-7. [DOI:10.1210/endo.137.7.8770941] [PMID] [DOI:10.1210/endo.137.7.8770941]
15. Hatami-Baroogh L, Razavi S, Zarkesh-Esfahani H, Tavalaee M, Tanhaei S, Ghaedi K, et al. Evaluation of the leptin receptor in human spermatozoa. Reproductive Biology and Endocrinology. 2010; 8(1):17. [DOI:10.1186/1477-7827-8-17] [PMID] [PMCID] [DOI:10.1186/1477-7827-8-17]
16. Yu WH, Walczewska A, Karanth S, McCann SM. Nitric oxide mediates leptin-induced Luteinizing Hormone-Releasing Hormone (LHRH) and LHRH and Leptin-Induced (LH) release from the pituitary gland. Endocrinology. 1997; 138(11):5055-8. [DOI:10.1210/endo.138.11.5649] [PMID] [DOI:10.1210/endo.138.11.5649]
17. Sone M, Nagata H, Takekoshi S, Osamura YR. Expression and localization of leptin receptor in the normal rat pituitary gland. Cell and Tissue Research. 2001; 305(3):351-6. [DOI:10.1007/s004410100407] [PMID] [DOI:10.1007/s004410100407]
18. Yu W, Kimura M, Walczewska A, Karanth S, McCann S. Role of leptin in hypothalamic-pituitary function. Proceedings of the National Academy of Sciences. 1997; 94(3):1023-8. [DOI:10.1073/pnas.94.3.1023] [DOI:10.1073/pnas.94.3.1023]
19. Lampiao F, Du Plessis SS. Insulin and leptin enhance human sperm motility, acrosome reaction and nitric oxide production. Asian Journal of Andrology. 2008; 10(5):799-807. [DOI:10.1111/j.1745-7262.2008.00421.x] [PMID] [DOI:10.1111/j.1745-7262.2008.00421.x]
20. Leal A, Caldas-Bussiere M, de Carvalho CP, Viana K, Quirino C. Role of nitric oxide on quality of freshly ejaculated bull spermatozoa during heparin-induced in vitro capacitation. Animal Reproduction Science. 2009; 116(1-2):38-49. [DOI:10.1016/j.anireprosci.2008.12.020] [PMID] [DOI:10.1016/j.anireprosci.2008.12.020]
21. Moretto M, Lopez F, Negro-Vilar A. Nitric oxide regulates luteinizing hormone-releasing hormone secretion. Endocrinology. 1993; 133(5):2399-402. [DOI:10.1210/endo.133.5.8104781] [PMID] [DOI:10.1210/endo.133.5.8104781]
22. Seilicovich A, Duvilanski B, Pisera D, Theas S, Gimeno M, Rettori V, et al. Nitric oxide inhibits hypothalamic luteinizing hormone-releasing hormone release by releasing gamma-aminobutyric acid. Proceedings of the National Academy of Sciences. 1995; 92(8):3421-4. [DOI:10.1073/pnas.92.8.3421] [DOI:10.1073/pnas.92.8.3421]
23. Revelli A, Bergandi L, Massobrio M, Lindblom B, Bosia A, Ghigo D. The concentration of nitrite in seminal plasma does not correlate with sperm concentration, sperm motility, leukocytospermia, or sperm culture. Fertility and Sterility. 2001; 76(3):496-500. [DOI:10.1016/S0015-0282(01)01958-6] [DOI:10.1016/S0015-0282(01)01958-6]
24. Abavisani A, Baghbanzadeh A, Shayan P, Tajik P, Dehghani H, Mirtorabi M. Leptin mRNA expresses in the bull reproductive organ. Veterinary Research Communications. 2009; 33(8):823-30. [DOI:10.1007/s11259-009-9230-7] [PMID] [DOI:10.1007/s11259-009-9230-7]
25. Glander HJ, Lammert A, Paasch U, Glasow A, Kratzsch J. Leptin exists in tubuli seminiferi and in seminal plasma. Andrologia. 2002; 34(4):227-33. [DOI:10.1046/j.1439-0272.2002.00501.x] [PMID] [DOI:10.1046/j.1439-0272.2002.00501.x]
26. Zorn B, Osredkar J, Meden‐Vrtovec H, Majdic G. Leptin levels in infertile male patients are correlated with inhibin B, testosterone and SHBG but not with sperm characteristics. International Journal of Andrology. 2007; 30(5):439-44. [DOI:10.1111/j.1365-2605.2006.00728.x] [PMID] [DOI:10.1111/j.1365-2605.2006.00728.x]
27. Chen B, Guo JH, Lu YN, Ying XL, Hu K, Xiang ZQ, et al. Leptin and varicocele‐related spermatogenesis dysfunction: Animal experiment and clinical study. International Journal of Andrology. 2009; 32(5):532-41. [DOI:10.1111/j.1365-2605.2008.00892.x] [PMID] [DOI:10.1111/j.1365-2605.2008.00892.x]
28. Steinman N, Gamzu R, Yogev L, Botchan A, Schreiber L, Yavetz H. Serum leptin concentrations are higher in azoospermic than in normozoospermic men. Fertility and Sterility. 2001; 75(4):821-2. [DOI:10.1016/S0015-0282(01)01707-1] [DOI:10.1016/S0015-0282(01)01707-1]
29. Kumar P, Saini M, Kumar D, Jan M, Swami DS, Sharma R. Quantification of leptin in seminal plasma of buffalo bulls and its correlation with antioxidant status, conventional and Computer-Assisted Sperm Analysis (CASA) semen variables. Animal Reproduction Science. 2016; 166:122-7. [DOI:10.1016/j.anireprosci.2016.01.011] [PMID] [DOI:10.1016/j.anireprosci.2016.01.011]
30. Banks WA, McLay RN, Kastin AJ, Sarmiento U, Scully S. Passage of leptin across the blood-testis barrier. American Journal of Physiology-Endocrinology and Metabolism. 1999; 276(6):E1099-E104. [DOI:10.1152/ajpendo.1999.276.6.E1099] [DOI:10.1152/ajpendo.1999.276.6.E1099]
31. Ellithy MMS, Shaeer OKZ, Gaafar KM. Correlation between leptin content and sperm retrieval in cases of functional azoospermia. The Journal of Basic & Applied Zoology. 2014; 67(5):164-72. [DOI:10.1016/j.jobaz.2013.10.004] [DOI:10.1016/j.jobaz.2013.10.004]
32. Aquila S, Gentile M, Middea E, Catalano S, Morelli C, Pezzi V, et al. Leptin secretion by human ejaculated spermatozoa. The Journal of Clinical Endocrinology & Metabolism. 2005; 90(8):4753-61. [DOI:10.1210/jc.2004-2233] [PMID] [DOI:10.1210/jc.2004-2233]
33. Lewis S, Donnelly E, Sterling E, Kennedy M, Thompson W, Chakravarthy U. Nitric oxide synthase and nitrite production in human spermatozoa: Evidence that endogenous nitric oxide is beneficial to sperm motility. Molecular Human Reproduction. 1996; 2(11):873-8. [DOI:10.1093/molehr/2.11.873] [PMID] [DOI:10.1093/molehr/2.11.873]
34. O'Bryan MK, Zini A, Cheng CY, Schlegel PN. Human sperm endothelial nitric oxide synthase expression: Correlation with sperm motility. Fertility and Sterility. 1998; 70(6):1143-7. [DOI:10.1016/S0015-0282(98)00382-3] [DOI:10.1016/S0015-0282(98)00382-3]
35. Zini A, O'Bryan MK, Magid MS, Schlegel PN. Immunohistochemical localization of endothelial nitric oxide synthase in human testis, epididymis, and vas deferens suggests a possible role for nitric oxide in spermatogenesis, sperm maturation, and programmed cell death. Biology of Reproduction. 1996; 55(5):935-41. [DOI:10.1095/biolreprod55.5.935] [PMID] [DOI:10.1095/biolreprod55.5.935]
36. Donnelly ET, Lewis S, Thompson W, Chakravarthy U. Sperm nitric oxide and motility: The effects of nitric oxide synthase stimulation and inhibition. Molecular Human Reproduction. 1997; 3(9):755-62. [DOI:10.1093/molehr/3.9.755] [PMID] [DOI:10.1093/molehr/3.9.755]
37. Zhang H, Zheng RL. Possible role of nitric oxide on fertile and asthenozoospermic infertile human sperm functions. Free Radical Research. 1996; 25(4):347-54. [DOI:10.3109/10715769609149057] [PMID] [DOI:10.3109/10715769609149057]
38. Revelli A, Soldati G, Costamagna C, Pellerey O, Aldieri E, Massobrio M, et al. Follicular fluid proteins stimulate Nitric Oxide (NO) synthesis in human sperm: A possible role for NO in acrosomal reaction. Journal of Cellular Physiology. 1999; 178(1):85-92. [DOI:10.1002/(SICI)1097-4652(199901)178:13.0.CO;2-Y] https://doi.org/10.1002/(SICI)1097-4652(199901)178:1<85::AID-JCP11>3.0.CO;2-Y [DOI:10.1002/(SICI)1097-4652(199901)178:13.0.CO;2-Y]
39. Rosselli M, Dubey RK, Imthurn B, Macas E, Keller PJ. Andrology: Effects of nitric oxide on human spermatozoa: Evidence that nitric oxide decreases sperm motility and induces sperm toxicity. Human Reproduction. 1995; 10(7):1786-90. [DOI:10.1093/oxfordjournals.humrep.a136174] [PMID] [DOI:10.1093/oxfordjournals.humrep.a136174]
40. Weinberg JB, Doty E, Bonaventura J, Haney A. Nitric oxide inhibition of human sperm motility. Fertility and Sterility. 1995; 64(2):408-13. [DOI:10.1016/S0015-0282(16)57743-7] [DOI:10.1016/S0015-0282(16)57743-7]
41. Schaad NC, Zhang XQ, Campana A, Schorderet-Slatkine S. Human seminal plasma inhibits brain nitric oxide synthase activity. Human Reproduction. 1996; 11(3):561-5. [DOI:10.1093/HUMREP/11.3.561] [PMID] [DOI:10.1093/HUMREP/11.3.561]
42. Köhn FM, Erdmann I, Oeda T, El Mulla K, Schiefer H, Schill WB. Influence of urogenital infections on sperm functions. Andrologia. 1998; 30(S1):73-80. [DOI:10.1111/j.1439-0272.1998.tb02829.x] [PMID] [DOI:10.1111/j.1439-0272.1998.tb02829.x]

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