Mon, Mar 17, 2025
Volume 2, Issue 2 (Summer 2004)
ASJ 2004, 2(2): 49-55 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mahmoud Zadeh Sagheb M R, Heidari Z, Rafigh Doost H, Sar Bishegi M. Study of Volumetric Changes of Rat's Embryonic Renal Glomeruli, Cortex and Medulla Following Lithium Carbonate Administration by Means of Stereological Method. ASJ 2004; 2 (2) :49-55
URL: http://anatomyjournal.ir/article-1-379-en.html
URL: http://anatomyjournal.ir/article-1-379-en.html
1- Histology Section, Anatomical Sciences Department, Zahedan Medical Sciences University, Zahedan, Iran.
Abstract: (1160 Views)
Purpose: To study the effects of lithium on the fetal rat's kidney and determine volumetric changes by an unbiased stereological method.
Materials and Methods: Thirty female and ten male Sprague dawley rats were chosen. Following cohabitation and observation of spermatozoa in the vaginal smear, the pregnant rats were divided into three groups (n=10). Treatment and control groups received 50 mg/kg lithium carbonate in distilled water and the same volume of normal saline by intraperitoneal injection on 7th, 10th, 13th, 16th and 19th days of gestation, respectively. On 20th day of gestation, under deep anesthesia, the right kidneys of the rats were dissected and fixed in modified Lillie's solution. Then tissue processing was performed, and 5-micrometer sections were cut and stained with H & E. To determine the volume parameters of the kidneys, a stereological technique based on Cavalier's principle was used.
Results: The study showed that lithium could cause a significant decrease in the glomerular volume (52%), total volume (44%) and volume of the cortex (42%) and medulla (49%) of the kidney.
Conclusion: Administration of lithium during pregnancy has effects on the volume of different parts of fetal kidneys, especially on glomeruli. Since normal volumetric parameters are indicators of renal development, it seems that lithium causes some defects in the normal development of the kidney.
Materials and Methods: Thirty female and ten male Sprague dawley rats were chosen. Following cohabitation and observation of spermatozoa in the vaginal smear, the pregnant rats were divided into three groups (n=10). Treatment and control groups received 50 mg/kg lithium carbonate in distilled water and the same volume of normal saline by intraperitoneal injection on 7th, 10th, 13th, 16th and 19th days of gestation, respectively. On 20th day of gestation, under deep anesthesia, the right kidneys of the rats were dissected and fixed in modified Lillie's solution. Then tissue processing was performed, and 5-micrometer sections were cut and stained with H & E. To determine the volume parameters of the kidneys, a stereological technique based on Cavalier's principle was used.
Results: The study showed that lithium could cause a significant decrease in the glomerular volume (52%), total volume (44%) and volume of the cortex (42%) and medulla (49%) of the kidney.
Conclusion: Administration of lithium during pregnancy has effects on the volume of different parts of fetal kidneys, especially on glomeruli. Since normal volumetric parameters are indicators of renal development, it seems that lithium causes some defects in the normal development of the kidney.
Type of Study: Original |
Subject:
Morphometry
Received: 2021/12/26 | Accepted: 2004/07/20 | Published: 2004/07/20
Received: 2021/12/26 | Accepted: 2004/07/20 | Published: 2004/07/20
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright © The Author(s);
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-By-NC), which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Contact Information
