Volume 20, Issue 2 (Summer & Autumn-In Press 2023)
ASJ 2023, 20(2): 0-0 |
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Khaledi S, Najafpour Pitka M, Hoseini Z S, Ghorbanlou M, Shirazi R. A Comparative Study Between N-acetyl Cysteine and Melatonin on Oocyte and Embryo Quality in Polycystic Ovary Syndrome. ASJ 2023; 20 (2)
URL: http://anatomyjournal.ir/article-1-224-en.html
URL: http://anatomyjournal.ir/article-1-224-en.html
Sajed Khaledi1 
, Maryam Najafpour Pitka2 , Zahra Sadat Hoseini3 , Mehrdad Ghorbanlou4 , Reza Shirazi5
, Maryam Najafpour Pitka2 , Zahra Sadat Hoseini3 , Mehrdad Ghorbanlou4 , Reza Shirazi5
1- Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
2- Department of Genetics, Tonekabon Islamic Azad University, Tonekabon, Iran.
3- Department of biology, Faculty of genetics, Arsanjan Islamic Azad University, Arsanjan, Iran.
4- Department of Anatomical Science, Iran University of Medical Science, Tehran, Iran.
5- Department of Anatomy, School of Medical Sciences, Biomedical & Health, UNSW Sydney, Sydney, Australia.
2- Department of Genetics, Tonekabon Islamic Azad University, Tonekabon, Iran.
3- Department of biology, Faculty of genetics, Arsanjan Islamic Azad University, Arsanjan, Iran.
4- Department of Anatomical Science, Iran University of Medical Science, Tehran, Iran.
5- Department of Anatomy, School of Medical Sciences, Biomedical & Health, UNSW Sydney, Sydney, Australia.
Abstract: (470 Views)
Background: Polycystic ovary syndrome (PCOS) often is associated with low-quality oocytes. The aim of the present study was to investigate the effects of N-acetyl cysteine (NAC), melatonin and their combination on follicular fluid (FF) parameters, oocytes and embryo quality in PCOS patients.
Methods: A prospective randomized placebo-controlled pilot study on 60 Iranian women with PCOS undergoing intracytoplasmic sperm injection (ICSI) was designed. Women were divided into four groups (n=15 in each): (1) melatonin group, administered 3 mg/day melatonin at 22:00; (2) NAC group, administered 1800 mg day-1 NAC; (3) NAC+melatonin group, and (4) placebo group. Drugs were administered from the 5th day of previous cycle until the day of oocyte aspiration (40 days treatment in total). Hormonal status, sperm parameters, oocyte morphology, fertilization rate and embryo quality were assessed. Data were analyzed by one-way ANOVA, with significance set at P≤0.05.
Results: The number of immature and abnormal oocytes decreased significantly in the NAC (1.27±0.88 (P=0.006) and 2.1±1.1 (P=0.001), respectively) compared with placebo group (3.33±1.35 and 3.7±1.7, respectively), with a concomitant increase in the number of good embryos (Grade I) in the NAC (2.87±1.45, P=0.02) in comparison to placebo (1.53±0.83) group. Insulin and luteinizing hormone (LH) levels were significantly lower in the melatonin (7±2.4 and 1.5±0.5, respectively) and NAC (7.1±2.5 and 1.5±0.5, respectively) groups (P=0.01) compared with the placebo-treated group (9.5±2.9 and 1.8±0.6, respectively).
Conclusion: NAC improves oocyte and embryo quality, but combination of NAC and melatonin do not apply a significant positive effect.
Methods: A prospective randomized placebo-controlled pilot study on 60 Iranian women with PCOS undergoing intracytoplasmic sperm injection (ICSI) was designed. Women were divided into four groups (n=15 in each): (1) melatonin group, administered 3 mg/day melatonin at 22:00; (2) NAC group, administered 1800 mg day-1 NAC; (3) NAC+melatonin group, and (4) placebo group. Drugs were administered from the 5th day of previous cycle until the day of oocyte aspiration (40 days treatment in total). Hormonal status, sperm parameters, oocyte morphology, fertilization rate and embryo quality were assessed. Data were analyzed by one-way ANOVA, with significance set at P≤0.05.
Results: The number of immature and abnormal oocytes decreased significantly in the NAC (1.27±0.88 (P=0.006) and 2.1±1.1 (P=0.001), respectively) compared with placebo group (3.33±1.35 and 3.7±1.7, respectively), with a concomitant increase in the number of good embryos (Grade I) in the NAC (2.87±1.45, P=0.02) in comparison to placebo (1.53±0.83) group. Insulin and luteinizing hormone (LH) levels were significantly lower in the melatonin (7±2.4 and 1.5±0.5, respectively) and NAC (7.1±2.5 and 1.5±0.5, respectively) groups (P=0.01) compared with the placebo-treated group (9.5±2.9 and 1.8±0.6, respectively).
Conclusion: NAC improves oocyte and embryo quality, but combination of NAC and melatonin do not apply a significant positive effect.
Type of Study: Original |
Subject:
Reproductive Biology
Received: 2018/07/3 | Accepted: 2024/09/11 | Published: 2023/08/30
Received: 2018/07/3 | Accepted: 2024/09/11 | Published: 2023/08/30
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