Volume 13, Issue 2 (Spring 2016 -- 2016)                   ASJ 2016, 13(2): 73-78 | Back to browse issues page

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1- Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
2- PhD Department of Food Hygiene & Quality Control, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran.
3- Department of Medical and Surgical Science, Faculty of Medicine, University of Bologna, Bologna, Italy.
Abstract:   (1004 Views)

Meat is rich in protein, minerals, and vitamins and therefore a main source of the human diet. Meat products are usually made from processed red meat and preservatives such as nitrate, to improve the overall quality of the food. Histologically, meat is mainly composed of skeletal muscle fibers, water, adipose and connective tissues. Growing bodies of evidence suggests that unauthorized ingredients or tissues in meat products are quite common, and this alters the product quality and safety. 
Histological methods make it possible to examine the composition of meat products directly; hence these methodologies are largely used to detect unpermitted herbal and animal contents. Histological and histometric methods are also used to test the percentage and quality of meat in meat products. Freezing and thawing meat cause formation of ice-crystals and muscle fiber modification. This can be identified using histological methodology. Moreover, the changes in pH and mineral content in meat products can be determined using the aforementioned approach. Overall, the histomorphological approach represents a reliable method to investigate the meat product contents and make customers and markets more certain about meat quality. This brief review is focused on the use of the histological method in the assessment of meat products.

Full-Text [PDF 622 kb]   (427 Downloads)    
Type of Study: Review |
Received: 2015/10/27 | Accepted: 2016/02/6 | Published: 2017/05/22

1. McNeill SH. Inclusion of red meat in healthful dietary patterns. Meat Science. 2014; 98(3):452-60. doi: 10.1016/j.meatsci.2014.06.028 [DOI:10.1016/j.meatsci.2014.06.028]
2. Binnie MA, Barlow K, Johnson V, Harrison C. Red meats: time for a paradigm shift in dietary advice. Meat Science. 2014; 98(3):445-451. doi: 10.1016/j.meatsci.2014.06.024 [DOI:10.1016/j.meatsci.2014.06.024]
3. Rohrmann S, Linseisen J. Processed meat: the real villain?. Proceedings of the Nutrition Society. 2015; 1-9. doi: 10.1017/S0029665115004255 [DOI:10.1017/S0029665115004255]
4. Heinz G, Hautzinger P. Meat processing technology for small to medium scale producers. Bangkok: RAP Publication; 2009.
5. Banks WJ. Applied veterinary histology. London: Mosby Publication; 1993.
6. Georgier L, Vitanov S. Adulteration of mince and sausages. Khranitelana Promish Lenost. 1995; 44(1):15-16.
7. Ballin NZ. Authentication of meat and meat products. Meat Science. 2010; 86(3):577–87. doi: 10.1016/j.meatsci.2010.06.001 [DOI:10.1016/j.meatsci.2010.06.001]
8. Institute of Standards and Industrial Research of Iran. [Detection of unauthorized animal tissue by histological method (Persian)] [Internet]. 2016 [Cited 16 March 2016]. Available from: http://www.isiri.gov.ir/Portal/file/?428116/6103.pdf
9. Lumley ID. Authenticity of meat and meat products. In: Ashurst P, Dennis MJ, editors. Food Authentication. New York: Springer Publication; 1996, p. 108-139. [DOI:10.1007/978-1-4613-1119-5_4]
10. Latorre R, Sadeghinezhad J, Hajimohammadi B, Izadi F, Sheibani MT. Application of morphological method for detection of unauthorized tissues in processed meat products. Journal of Food Quality & Hazards Control. 2015; 2(2):71-74. doi: 10.17221/167/2015-cjfs [DOI:10.17221/167/2015-CJFS]
11. Prayson B, McMahon JT, Prayson RA. Fast food hamburgers: what are we really eating? Annals of Diagnostic Pathology. 2008a; 12(6):406-409. doi: 10.1016/j.anndiagpath.2008.06.002 [DOI:10.1016/j.anndiagpath.2008.06.002]
12. Prayson BE, McMahon JT, Prayson RA. Applying morphologic techniques to evaluate hotdogs; what is in the hotdogs we eat? Annals of Diagnostic Pathology. 2008b; 12(6):98-102. doi: 10.1016/j.anndiagpath.2007.04.012 [DOI:10.1016/j.anndiagpath.2007.04.012]
13. Jahed-Khaniki GR, Rokni N. [Histological study of unpermitted tissues in heated meat products by using of Masson's trichrome stain (Persian)]. Animal Sciences (Pajouhesh & Sazandegi). 2007; 73(4):96-102.
14. Sadeghinezhad J, Hajimohammadi B, Izadi F, Yarmahmoudi F, Latorre R. Evaluation of the morphologic method for the detection of animal and herbal content in minced meat. Czech Journal of Food Science. 2015; 33(6):564-69. doi: 10.17221/167/2015-cjfs [DOI:10.17221/167/2015-CJFS]
15. Jahed-Khaniki GR, Rokni N. [Histological detection of soya in freezing raw hamburger of Iran (Persian)]. Animal Sciences (Pajouhesh & Sazandegi). 2004; 62(1):71-75.
16. Izadi F, Sadeghinezhad J, Hajimohammadi B, Taghipour-zahir SH, Fallahzadeh H, Sheibani MT, et al. [Detection of Unauthorized Tissues in Trade Frozen Minced Meat Marketed in Yazd with Histological Method (Persian)]. Toloo-e Behdasht. 2015; 14(6):423-431.
17. Rokni N, Rezaian M, Dayani Dardashti A. [Histological histometrical study of different heated sausages (Persian)]. Journal of Veterinary Medicine. 1997; 52(1):95-103.
18. Botka-Petrak K, Hraste A, Lucić H, Gottstein Ž, Gomerčić MĐ, Jakšić S, et al. Histological and chemical characteristics of mechanically deboned meat of broiler chickens. Veterinarski Archive. 2011; 81(2):273-283.
19. Ghisleni G, Stella S, Radaelli E, Mattiello S, Scanziani E. Qualitative evaluation of tortellini meat filling by histology and image analysis. International Journal of Food Science & Technology. 2010; 45(2):265-70. doi: 10.1111/j.1365-2621.2009.02130.x [DOI:10.1111/j.1365-2621.2009.02130.x]
20. Sadeghi E, Khazaei M, Almasi A, Shariatifar N, Bohlouli Oskoii S, Tahvilian N. [Recognition of illegal tissues in the meat products from Kermanshah supply centers during the years 2009-2010 (Persian)]. Ofogh-e Danesh. 2011; 17(1):55-59.
21. Abbasy-Fasarani M, Hosseini H, Jahed-Khaniki GR, Adibmoradi M, Eskandari S. [Histological study of industrial hamburgers containing 30 and 60 percent meat for presence of unpermitted edible tissues and correlation of this factor to meat connective tissue chemical indices (Persian)]. Iranian Journal of Nutrition Sciences & Food Technology. 2012; 7(5):311-318.
22. Fekri M, Hosseini H, Eskandari S, Jahed-Khaniki GR, Adib-Moradi M. [Histological study of sausages in point of unpermitted edible tissues assessment and its relationship to collage and hydroxyprolin of product (Persian)]. Iranian Journal of Nutrition Sciences & Food Technology. 2013; 10(41):107-116.
23. Tersteeg MH, Koolmees PA, van Knapen F. Im­munohistochemical detection of brain tissue in heated meat products. Meat Science. 2002; 61(1):67-72. doi: 10.1016/s0309-1740(01)00164-4 [DOI:10.1016/S0309-1740(01)00164-4]
24. Herde K, Bergmann M, Lang C, Leiser R, Wenisch S. Glial fibrillary acidic protein and myelin basic protein as markers for the immunochemical detection of bovine central nervous tissue in heat-treated meat products. Journal of Food Protection. 2005; 68(4):823-827. [DOI:10.4315/0362-028X-68.4.823] [PMID]
25. Wenisch S, Lücker E, Eigenbrodt E, Leiser R, Bülte M. Detection of central nervous tissue in meat prod­ucts – An immunohistochemical approach. Nutrition Research. 1999; 19(8):1165-172. doi: 10.1016/s0271-5317(99)00077-9 [DOI:10.1016/S0271-5317(99)00077-9]
26. Izadi F, Sadeghinezhad J, Hajimohamadi B, Sheibani MT. [Efficacy of histological examination in detection of fraud in minced meat (Persian)]. Journal of Health. 2016; 7(4):386-94.
27. Tremlova B, Starha P. Histometric evaluation of meat products-determination of size and number of objects. Czech Journal of Food Sciences. 2002; 20(5):175-180.
28. Tremlova B, Starha P. Histometric evaluation of meat products: Determination of area and comparison of results obtained by histology and chemistry. Czech Journal of Food Sciences. 2003; 21(3):101-106.
29. Rencova E. Microscopic method and image analysis of starch in food. Paper presented at: 8th Symposium of Zouny Research Wolker. 2007; Brno, Czech.
30. Pospiech M, Tremlová B, Renčová E, Randulová Z. Immunohistochemical detection of soya protein–Optimisation and verification of the method. Czech Journal of Food Sciences. 2009; 27(1):11-19.
31. Zhu S, Bail A, Ramaswamy HS, Chapleau N. Characterization of ice crystals in pork muscle formed by pressure-shift freezing as compared with classical freezing methods. Journal of Food Science. 2004; 69(4):190-197. doi: 10.1111/j.1365-2621.2004.tb06346.x [DOI:10.1111/j.1365-2621.2004.tb06346.x]
32. Martino MN, Zaritzky NE. Ice crystal size modifications during frozen beef storage. Journal of Food Science. 1988; 53(6):1631-37. doi: 10.1111/j.1365-2621.1988.tb07802.x [DOI:10.1111/j.1365-2621.1988.tb07802.x]
33. Martino MN, Otero L, Sanz PD, Zaritzky NE. Size and location of ice crystals in pork frozen by high-pressure-assisted freezing as compared to classical methods. Meat Science. 1998; 50(3):303-13. doi: 10.1016/s0309-1740(98)00038-2 [DOI:10.1016/S0309-1740(98)00038-2]
34. Icier F, Izzetoglu GT, Bozkurt H, Ober A. Effects of ohmic thawing on histological and textural properties of beef cuts. Journal of Food Engineering. 2010; 99(3):360-365. doi: 10.1016/j.jfoodeng.2010.03.018 [DOI:10.1016/j.jfoodeng.2010.03.018]
35. Anese M, Manzocco L, Panozzo A, Beraldo P, Foschia M, Nicoli MC. Effect of radiofrequency assisted freezing on meat microstructure and quality. Food Research International. 2012; 46(1):50-54. doi: 10.1016/j.foodres.2011.11.025 [DOI:10.1016/j.foodres.2011.11.025]
36. Sharedeh D, Gatellier PH, Astruc TH, Daudin JD. Effects of pH and NaCl levels in a beef marinade on physicochemical states of lipids and proteins and on tissue microstructure. Meat Science. 2015; 110:24-31. doi: 10.1016/j.meatsci.2015.07.004 [DOI:10.1016/j.meatsci.2015.07.004]
37. Astruc TH, Labas R, Vendeuvre JL, Martin JL, Taylor RG. Beef sausage structure affected by sodium chloride and potassium lactate. Meat Science. 2008; 80(4):1092-1099. doi: 10.1016/j.meatsci.2008.05.007 [DOI:10.1016/j.meatsci.2008.05.007]
38. Laville E, Sayd TH, Sante´-Lhoutellier V, Morzel M, Labas R, Franck M, et al. Characterisation of PSE zones in semimembranosus pig muscle. Meat Science. 2005; 70(1):167-172. doi: 10.1016/j.meatsci.2004.12.008 [DOI:10.1016/j.meatsci.2004.12.008]