Anatomical Sciences Journal
مجله علوم تشریح
ASJ
Basic Sciences
http://anatomyjournal.ir
1
admin
17286158
17286158
en
jalali
1390
8
1
gregorian
2011
11
1
9
36
online
1
fulltext
fa
Comparison of the effect of Growth Factors on the Development of Pre-implantation Embryos of Vitrified Mouse
Morphometry
Morphometry
Original
Original
<strong>Purpose</strong>: To improve the development of frozen mouse embryos by adding fibroblast growth factors (FGF) and hepatocytes (HGF) to the culture medium.<strong> Materials and Methods</strong>: Two-cell mouse embryos obtained from the fallopian tube and frozen by cryotopic method were treated in T6 medium with a dose of 20 ng / ml FGF and 20 ng / ml HGF. The results of their culture were compared with control groups and the rate of embryonic development was evaluated.<strong>Results</strong>: The results showed that the rate of cleavage and development of vitrified and non-vitrified mouse embryos in the culture medium by adding growth factors was significantly increased compared to the control group (p <0.01). There was also a significant difference in the rate of morula development between vitrified and non-vitrified embryos treated with growth factors (p <0.05), but this difference was not observed in the rate of blastocyst formation (p> 0.05). <strong>Conclusion:</strong> The results of this study show that the addition of growth factors in the culture medium improves the pre-implantation development of mouse embryos after vitrification.
Growth Factors, Pre-implantation Development, Vitrification
0
0
http://anatomyjournal.ir/browse.php?a_code=A-10-304-237&slc_lang=fa&sid=1
Fatemeh
Ghasemian
10031947532846006228
10031947532846006228
No
Mahnaz
Azarnia
10031947532846006229
10031947532846006229
No
Masoumeh
Faghani Langroudi
10031947532846006230
10031947532846006230
No
Mohammad Hadi
Bahadori
10031947532846006231
10031947532846006231
Yes