Anatomical Sciences Journal
مجله علوم تشریح
ASJ
Basic Sciences
http://anatomyjournal.ir
1
admin
17286158
17286158
en
jalali
1389
8
1
gregorian
2010
11
1
8
32
online
1
fulltext
fa
The Evaluation of Melatonin Effect on In-Vitro Development of Mouse Preimplantation Embryos
Morphometry
Morphometry
Original
Original
<strong>Purpose</strong>: Melatonin promotes in-vitro embryo development in different species. This study studied the effects of melatonin on in-vitro mouse preimplantation embryo development. <strong>Materials and Methods</strong>: Two-cell embryos were obtained from oviduct of 6-8 weeks female NMRI mice 48 hours after administration of an intra-peritoneal injection of 5 IU/ml pregnant mares’ serum gonadotrophin and subsequent 5 IU/ml human chorionic gonadotrophin (ip). Embryos were cultured in T6 culture medium supplemented with different dosages of melatonin {0 (control group), 100×10-6 M, 10×10-6 M, 1×10-6 M, 100×10-9 M and 10×10-9 M (1-5 treatment groups)}. With due attention to percent of embryos in different stages, the rate of development of embryos was assessed using of invert microscope. It is also compared to control group. <strong>Results</strong>: The results showed that the rate of cleavage and development of mouse the embryos to blastocyst stage increased significantly in the development culture medium supplemented with 10 and 100 nM/mg of melatonin in comparison to control group (p<0.001). <strong>Conclusion</strong>: The results of this study demonstrate that, enriching the culture medium with melatonin, improve mouse preimplantation development.
Melatonin, Preimplantation Embryo, Development, Mouse
0
0
http://anatomyjournal.ir/browse.php?a_code=A-10-304-214&slc_lang=fa&sid=1
Zakieh
Asgari
10031947532846006926
10031947532846006926
No
Mina
Ramezani
10031947532846006927
10031947532846006927
No
Mohammad Hadi
Bahadori
10031947532846006928
10031947532846006928
Yes
Masoumeh
Ahmadi Jalali Moghaddam
10031947532846006929
10031947532846006929
No