Anatomical Sciences Journal
مجله علوم تشریح
ASJ
Basic Sciences
http://anatomyjournal.ir
1
admin
17286158
17286158
en
jalali
1388
5
1
gregorian
2009
8
1
7
27
online
1
fulltext
fa
Buserelin Inhibits Apoptosis in Male Germ Cells Induced by Busulfan in Mouse Testis
Morphometry
Morphometry
Original
Original
<strong>Purpose</strong>: The aim of this study was to investigate the effect of buserelin on apoptosis of male germ cells induced by busulfan in adult male mice. <strong>Materials and Methods</strong>: Male adult NMRI mice were divided into four group of eight each. Group 1 (control) administered PBS for 21 days subcutaneously, group 2 given 0.4 mg buserelin for 21 days subcutaneously, group 3 given single dose of 30 mg/kg busulfan intraperitoneally and group 4 given both busulfan and buserelin for 21 days. The animals were sacrificed and their testes were dissected 35 days after the treatment. Evaluations were made by determining Johnson's score and apoptosis were assayed by terminal- deoxynucleotidyl- transferase-mediated dutp nick end labeling (TUNEL). Statistical analyses were performed using ANOVA test. <strong>Results</strong>: Recovery status and Johnson's score in group 4were significantly higher than those of busulfan treated group 7.71±0.69 VS 4.46±0.56 (p< 0.001). Apoptotic cells number cells were significantly more numerous in busulfan treated group than those of control 23.28±7.10 VS 3.54±1.02 (p<0.001). While buserelin substantially reduced germ cell apoptosis in fourth group 10.50±2.91 in comparison with third group 23.28±7.10, (p<0.001).<strong>Conclusion:</strong> Administration of buserelin after testicular damage by busulfan enhances the regeneration of spermatogenesis in mouse through inhibition of apoptosis in germ cells.
Testis, Spermatogenesis, Apoptosis, Buserelin
0
0
http://anatomyjournal.ir/browse.php?a_code=A-10-304-182&slc_lang=fa&sid=1
Fahimeh
Mohammad Ghasemi
10031947532846005963
10031947532846005963
Yes
Mohammad Hadi
Bahadori
10031947532846005964
10031947532846005964
No
Masoumeh
Faghani Langeroudi
10031947532846005965
10031947532846005965
No
Ebrahim
Nasiri
10031947532846005966
10031947532846005966
No
Jaffar
Soleymanirad
10031947532846005967
10031947532846005967
No