Anatomical Sciences Journal
مجله علوم تشریح
ASJ
Basic Sciences
http://anatomyjournal.ir
1
admin
17286158
17286158
en
jalali
1386
2
1
gregorian
2007
5
1
5
18
online
1
fulltext
fa
The Effects of Lithium Chloride on Survival and Function of in Vitro Cultured Human Granulosa Cells
Morphometry
Morphometry
Original
Original
<strong>Purpose</strong>: To investigate the effect of lithium chloride (LiCl) on survival and functional activity of in vitro cultured human granulosa cells (GCs).<br>
<strong>Materials and Methods</strong>: GCs were isolated from follicular fluid of women undergoing IVF/ICSI. After RBCs removal, GCs were plated in a medium containing varying concentrations of LiCl including 1, 2 and 5mM for two weeks period. A culture without LiCl was taken as control group. To induce apoptosis, the medium of all cultures, on day 14 were replaced by serum free media and the culture was extended for further 24 hour. At the end of cultivation period, apoptosis was assessed by TUNEL and flowcytometry analysis. Furthermore to assess secretional function of the cells in various groups, in some cultures, FSH and androstondion hormones were added and after 48 hours, subsequent secretion of 17-b estradiol was determined by RIA analysis.<br>
<strong>Results</strong>: The result showed that in GCs treated with 2 and 5 mM LiCl, apoptosis was statistically decreased more than 50% compared to control group (P>0.05) and estradiol secretion increased more than 40% in the GCs treated with 1,2,5 mM LiCl compared to control group.<br>
<strong>Conclusion</strong>: These results suggest that lithium increase functional activity of GCs and can protect GCs against apoptosis.
Granulosa cells, Lithium chloride, Apoptosis, 17-beta estradiol
37
47
http://anatomyjournal.ir/browse.php?a_code=A-10-304-158&slc_lang=fa&sid=1
Bahareh
Beyki
10031947532846006443
10031947532846006443
No
Poopak
Eftekhari Yazdi
10031947532846006444
10031947532846006444
Yes
Embryology Department, Royan Institute, Tehran, Iran.
Bahman
Zeynali
10031947532846006445
10031947532846006445
No
mojtaba
Reza Zadeh Valoujerdi
10031947532846006446
10031947532846006446
No
Leyli
Karimian
10031947532846006447
10031947532846006447
No
Reza
Salman Yazdi
10031947532846006448
10031947532846006448
No
Ehsan
Taghi Abadi
10031947532846006449
10031947532846006449
No