Anatomical Sciences Journal
مجله علوم تشریح
ASJ
Basic Sciences
http://anatomyjournal.ir
1
admin
17286158
17286158
en
jalali
1384
8
1
gregorian
2005
11
1
3
3
online
1
fulltext
fa
Molecular Cloning and Mutagenesis of Rat Glucocerebrosidase Gene
Morphometry
Morphometry
Original
Original
<strong>Purpose</strong>: The aim of this study was cloning the Gba enzyme in pUCBM21 plasmid, and making frame mutation on it and sequencing it.<br>
<strong>Materials and methods</strong>: mRNA was extracted from mouse spleen and glucocerebrosidase cDNA was synthesized and amplified by PCR with specific primers. cDNA was cloned in pUCBM21 and analyzed by restriction enzymes. A fragment of its sequence was deleted using MscI restriction enzyme .Then frame mutation was prepared and sequenced.<br>
<strong>Results</strong>: glucocerebrosidase cDNA was synthesized and amplified by PCR. Then cloned in pUCBM21 and confirmed using restriction analysis. An in frame mutation was done and confirmed by sequencing.<br>
<strong>Conclusion</strong>: The successful sequenced Gba cDNA compared with other researchers results.
Glucocerebrosidase, Cloning, Pucbm21 in frame mutation
185
190
http://anatomyjournal.ir/browse.php?a_code=A-10-304-109&slc_lang=fa&sid=1
Homayoun
Naderian
10031947532846006193
10031947532846006193
Yes
Anatomy Department, Medical Facalty, Kashan Medical Sciences University, Kashan, Iran.
Bahram
Kazemi
10031947532846006194
10031947532846006194
No
Ahmad
Hoseini
10031947532846006195
10031947532846006195
No
Yoosef
Sadeghi
10031947532846006196
10031947532846006196
No
Antovan
De Vries
10031947532846006197
10031947532846006197
No