Anatomical Sciences Journal
مجله علوم تشریح
ASJ
Basic Sciences
http://anatomyjournal.ir
1
admin
17286158
17286158
en
jalali
1382
11
1
gregorian
2004
2
1
1
5
online
1
fulltext
fa
Lectin Histochemistry Assessment of Seminiferous Epithelium Glycoconjugates in Mouse
Morphometry
Morphometry
Original
Original
<strong>Purpose</strong>: Plasma membrane glycoconjugates are important determinantsof differentiation and iteraction during spermtogenesis. Which entails miotic and meiosis proliferation, alternation in cell shape and so on. Hihg rates of them synthetized and present in acrosomes. With attention to such importancy, the present study has was designed to analyze glycoconjugates with special terminal sugar residues by meanes of lectin histochemistry.<br>
<strong>Materials and Methods</strong>: samples abtained from fifteen healthy fertile mice. After fixation and routine laboratory process, 5,µm sections prepared from paraffinian blocks. Lectin histochmistry was used to perform in situ characterization glycoconjugates present in mouse seminiferoustubules.<br>
<strong>Results</strong>: There wasnt any reaction in spermatogonia. Reaction to Soybean, Madura pamifera. Arachis hypogaea and Vicia villosa agglutinin lectins apeared in cytoplasm,plasmamembrane, preacrosomal granules in first spermatocyte and increased in early spermatids. This followed by reduction in late spermatids. There wasnt any affinity for Lotus tetragonolobus agglutinin in germ cells. Sertoli cells reacted with Maclura parnifera and Arachis hypogaea agglutinin lectins.<br>
<strong>Conclusion</strong>: As terminl sugars in a high quantity, galactose and N-acetylgalactosamine. in acrosome, cytoplasm and plasmamembrane have possible and important role in spermatogenesis. In late spermatid for protection these sugars may mask sialic acid and therefore diminish reactions. Acrosome was the most relevant lectin-labeled structure.
Spermatogenesis, Lectin, Glycoconjugate,Testis
29
37
http://anatomyjournal.ir/browse.php?a_code=A-10-304-43&slc_lang=fa&sid=1
Farzaneh
Zaman Soltani
10031947532846006751
10031947532846006751
Yes
Mashhad Medical Sciences University, Mashhad, Iran.
Ali Reza
Mahmoudian
10031947532846006752
10031947532846006752
No
Seyyed Hasan
Alavi
10031947532846006753
10031947532846006753
No
Ali Reza
Fazel
10031947532846006754
10031947532846006754
No