Anatomical Sciences Journal
مجله علوم تشریح
ASJ
Basic Sciences
http://anatomyjournal.ir
1
admin
17286158
17286158
en
jalali
1382
8
1
gregorian
2003
11
1
1
4
online
1
fulltext
fa
Inhibition of Microsome-Mediated Binding of Benzo (Α) Pyrene to "Dna By Cytosolic Reaction From Liver And Skin Rats in Cvitro
Morphometry
Morphometry
Original
Original
<strong>Purpose</strong>: The aim of this study was to evaluate the effect of age on the capacity of liver and epiderm of adult and weanging rats in transformation of Benzo (α) Pyrene.<br>
<strong>Materials and Methods</strong>: In a metabolic activiation assay system, cytochorome P-50 (from microsomal fraction) catalyses the formation of reactive epoxide of BaP which can then interact with exogenous DNA The capacity of cytochrome P-450 for BαP-DNA adducts formation from different organs are adult rat live>>> adult rat skin>> weanging rat liver> weanling rat skin. With the addition of cytosol (the source of glutathione S transferase (GST) to the microsome-mediated system an In vitro detoxification model has been established.<br>
<strong>Results</strong>: The results obtained by the addition of different cytosolic samples are shown in several tables in the text. These data show that isolation of subcellular fractions from young rats are less efficient in the biotransformation of BαP and detoxification by GSH is a passive process that depends on activation process. Also metabolic activation of BaP with cytochorome P-450 is not the first degree with glutathione detoxification. However, the results obtined In vitro do not reflect the exact change In vivo.
Benzo (Α) pyrene, Dna, Glutathione, Rat
53
62
http://anatomyjournal.ir/browse.php?a_code=A-10-304-36&slc_lang=fa&sid=1
Mostafa
Mohammadi
10031947532846006727
10031947532846006727
Yes
Biochemistry and Microbiology Departmen, Islamic Azad University, Flavarjan, Iran.
Abdol Amir
Allameh
10031947532846006728
10031947532846006728
No
Mohammad Hassan
Asadi Sar Yazdi
10031947532846006729
10031947532846006729
No
Mohammad Reza
KHhajeh
10031947532846006730
10031947532846006730
No