Anatomical Sciences Journal
مجله علوم تشریح
ASJ
Basic Sciences
http://anatomyjournal.ir
1
admin
17286158
17286158
en
jalali
1391
11
1
gregorian
2013
2
1
10
1
online
1
fulltext
en
Study of the Effect of Hypothyroidism on the Apoptotic Index in Rat Ovarian Follicles, Using the TUNEL Technique
Study of the Effect of Hypothyroidism on the Apoptotic Index in Rat Ovarian Follicles, Using the TUNEL Technique
Original
Original
<strong>Introduction:</strong> Among the endocrine diseases, hypothyroidism is the commonest after diabetes.Thyroid hormones (T3,T4) are essential for genital organs function. Apoptosis process in ovarian cells plays a significant role of development in ovarian follicles. The aim of the present study was determine the apoptotic index induced by hypothyroidism in rat ovarian follicles. <p><strong>Materials and Methods:</strong> In the present study, twenty female mature wistar rats were used with age of 2.5 months and weight of 200-250 (g). Rats were divided into test and control groups. In test group chemical hypothyroidism induced by propylthiouracil (PTU500 mg/L) in drinking water. The control group only received normal drinking water. After three weeks the rats were killed and their ovaries removed and were fixed for tissue preparation. TUNEL technique were used for determine of apoptosis. Cells count done by stereological method. Data were analyzed by t-test and one-way ANOVA followed by Tukey test. Significance was accepted at P<0.05. </p><p><strong>Results:</strong> The findings showed that the apoptotic index had a significant decrease in late antral and graffian follicles (P=0.000) and no significant decrease in preantral and early antral follicles (P>0.05) in hypothyroid group. All granulosa cells were TUNEL-positive in primary follicles but no cell was seen in primordial follicles in groups. </p><p><strong>Conclusion:</strong> The results of the present study showed that the hypothyroidism may be vigorous decreased of apoptotic index in antral and graffian follicles. Hypothyroidism increased the number of luteal bodies and decreased the number of graffian follicles in ovarian tissue. </p>
<p>
<strong>Introduction:</strong> Among the endocrine diseases, hypothyroidism is the commonest after diabetes.Thyroid
hormones (T3,T4) are essential for genital organs function. Apoptosis process in ovarian cells plays a
significant role of development in ovarian follicles. The aim of the present study was determine the apoptotic
index induced by hypothyroidism in rat ovarian follicles.
</p><p><strong>Materials and Methods:</strong> In the present study, twenty female mature wistar rats were used with age of 2.5
months and weight of 200-250 (g). Rats were divided into test and control groups. In test group chemical
hypothyroidism induced by propylthiouracil (PTU500 mg/L) in drinking water. The control group only
received normal drinking water. After three weeks the rats were killed and their ovaries removed and were
fixed for tissue preparation. TUNEL technique were used for determine of apoptosis. Cells count done by
stereological method. Data were analyzed by t-test and one-way ANOVA followed by Tukey test.
Significance was accepted at P<0.05.
</p><p><strong>Results:</strong> The findings showed that the apoptotic index had a significant decrease in late antral and graffian
follicles (P=0.000) and no significant decrease in preantral and early antral follicles (P>0.05) in hypothyroid
group. All granulosa cells were TUNEL-positive in primary follicles but no cell was seen in primordial
follicles in groups.
</p><p><strong>Conclusion:</strong> The results of the present study showed that the hypothyroidism may be vigorous decreased of
apoptotic index in antral and graffian follicles. Hypothyroidism increased the number of luteal bodies and
decreased the number of graffian follicles in ovarian tissue.
</p>
Hypothyroidism, Apoptosis, Ovarian Follicle, Thyroid Gland, Rats
Hypothyroidism, Apoptosis, Ovarian Follicle, Thyroid Gland, Rats
25
36
http://anatomyjournal.ir/browse.php?a_code=A-10-25-12&slc_lang=en&sid=1
Mahmoud
Moghaddam-Dorafshani
1003194753284600184
1003194753284600184
No
Medi
Jalali
1003194753284600185
1003194753284600185
Yes
Mohammad Reza
Nikravesh
1003194753284600186
1003194753284600186
No
Ali Reza
Ebrahimzadeh
1003194753284600187
1003194753284600187
No