Anatomical Sciences Journal
مجله علوم تشریح
ASJ
Basic Sciences
http://anatomyjournal.ir
1
admin
17286158
17286158
en
jalali
1381
11
1
gregorian
2003
2
1
1
1
online
1
fulltext
fa
Investigation Of Teratogenic Effect Of Methoxsalen On Developing Mouse Embryos Kidney And Liver In Days 7,8 And 9 Gestation
Morphometry
Morphometry
Original
Original
<strong>Introduction</strong>: Psoralens are a class of three ring, heterocyclic and planar compounds that exist naturally in a variety of plants and are used in the treatment of a number of epidermal proliferative disorders. The methoxsalen with formula of 9-methoxy-7H furo-[3,2-g][1] benzopyron-7-one belongs to this group. To study the probable effects of methoxsalen drug on developing mouse embryos.<br>
<strong>Materials & Methods</strong>: In this study the methoxsalen was applied alone and together with longwave ultraviolet rays (320-400 nm, UVA) into pregnant Balb/c mice. The standard of LD50 was determind 300 mg/kg and the UVA dose which causes erythema was calculated 0.048 j/cm2. A sublethal dose of 160 mg/kg of methoxsalen solution was injected intraperitoneally to pregnant mice on days 7,8 and 9 of gestation. then, after 2 hours the mice were exposed to UVA radiation of 0.034 j/cm2. Experiments included methoxsalen with UVA and UVA alone.<br>
<strong>Results</strong>: The macroscopic and microscopic studies on the embryos in days 15 of gestation showed significant anomalies at P<0.05 among the experimental embryos compared with the control individuals. In the experimental embryos anomalies included decrease of hepatocytes increase of megacaryocytes increase of nucleated erythrocytes decrease of glomerules and renal tubules numbers.<br>
<strong>Conclusion</strong>: This investigation shows the embryonic defects of methoxsalen during te period of organogenesis.
Methoxsalen, Longwave ultraviolet rays (320-400 Nm,Uva), Teratogenesis, Mouse embryo
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http://anatomyjournal.ir/browse.php?a_code=A-10-304-9&slc_lang=fa&sid=1
Parivar
Kazem
10031947532846006627
10031947532846006627
Yes
Sciences Faculty, Teacher Education University, Tehran, Iran.
Mohsen
Fadaei
10031947532846006628
10031947532846006628
No
Abbas
Shokravi
10031947532846006629
10031947532846006629
No