Anatomical Sciences Journal
مجله علوم تشریح
ASJ
Basic Sciences
http://anatomyjournal.ir
1
admin
17286158
17286158
en
jalali
1399
11
1
gregorian
2021
2
1
18
1
online
1
fulltext
en
P96 Technique for Tissue Plastination in Preparing Long-Lasting Anatomical Specimens
Plastination
Plastination
Original
Original
<div style="text-align: justify;"><strong>Introduction</strong>: Plastination is a technique of long-term preservation of the natural tissues without the typical problems related to wet specimens. Plastinated specimens are durable, dry, and flexible. In this process, the water and fat in biological tissues are substituted by a curable polymeric resin, i.e., subsequently cured. In this study, a new unsaturated polyester resin (P96) was synthesized and formulated to improve plastination techniques. These approaches were applied in the plastination laboratory at Isfahan Medical School based on our previous synthesized resin (P87) and the commercial resin (P35).<br>
<strong>Methods</strong>: P96 was formulated using different polyols, saturated, and unsaturated poly-acids, as the ploy esterification monomers. Then, the resulted pre-polymer was cured by the reaction of a vinyl monomer (hardening agent) in the presence of a radical initiator (hardening promoter) to form a tough durable film. The components of the resin were finely tuned to achieve the desired properties for organ plastination. Then, forced impregnation in a vacuum chamber was performed in the presence of P96 at 25oC following the conventional protocol, fixation, slicing (if necessary), dehydration, and defatting (if necessary). Finally, the curing (hardening) of P96 specimens was achieved by UVA-light irradiation.<br>
<strong>Results</strong>: According to the mechanical test results, P96 presented higher tensile strain and flexibility than P35 and P87. P87 had higher bending capability, compared to P35 and P96. P96 specimens were lighter, compared to P35 and P87 specimens. P96 specimens were more odorless than P87 specimens. In the naked survey of specimens, P96 specimens presented a better appearance concerning natural color and texture for plastinated specimens as well as the resin uniformity, consistency, and transparency of cadaveric tissues.<br>
<strong>Conclusion</strong>: The current study introduced a novel plastination method using a new formulated resin, P96. This technique is a quick and less expensive method for producing sheet as well as bulk plastinated specimens of different organs, i.e., appropriate in teaching anatomical sciences. P96 can be considered an upgraded resin, i.e., transparent and flexible with an easy and controllable hardening process. It has resolved the deficiencies of previous polyester resins. </div>
Organ preservation, Anatomy, Unsaturated polyester resin, Tissue plastination
31
44
http://anatomyjournal.ir/browse.php?a_code=A-10-121-2&slc_lang=en&sid=1
Fatemeh
Rafiemanzelat
frafiemanzelat@sci.ui.ac.ir
10031947532846003997
10031947532846003997
No
Department of Chemistry, Organic Polymer Chemistry Research Laboratory, University of Isfahan, Isfahan, Iran.
Mehdi
Sheikhi
sheikhi.m89@gmail.com
10031947532846003998
10031947532846003998
No
Department of Chemistry, Organic Polymer Chemistry Research Laboratory, University of Isfahan, Isfahan, Iran.
Abbas Ali
Rabiei
ab_rabiei@med.mui.ac.ir
10031947532846003999
10031947532846003999
Yes
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran.
Mohsen
Setayeshmehr
m_setayeshmehr@med.mui.ac.ir
10031947532846004000
10031947532846004000
No
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran.