Anatomical Sciences Journal
مجله علوم تشریح
ASJ
Basic Sciences
http://anatomyjournal.ir
1
admin
17286158
17286158
en
jalali
1397
11
1
gregorian
2019
2
1
16
1
online
1
fulltext
en
Investigating Morphologic Changes and Viability of Rats’ Bone Marrow Mesenchymal Stem Cells in Microgravity
Stem Cell
Stem Cell
Original
Original
<div style="text-align: justify;"><strong>Introduction: </strong>Mesenchymal Stem Cells (MSCs) are multipotent cells capable of duplication and auto-recovery and distinction from various cells including chondrocytes, adipocytes, chondroblasts, fibroblasts, and osteoblasts. Human stem cells are always subject to local and external mechanical loads. External loads are caused by physical activity in external environment loading to infliction of static and dynamic loads on the body and internal loads are typically caused due to body physiological function. Mechanical factors can affect different parameters such as morphology, proliferation, migration, metabolism and death as well as chemical changes in cells and lead to chemical changes in extracellular matrix and intracellular environment, besides distinction of cells. <br>
<strong>Methods:</strong> MSCs were isolated from rat’s bone marrow, then cultured in microgravity conditions. Morphologic changes of cells were analyzed by taking pictures at different times.<br>
<strong>Results:</strong> Results indicated a reduction in cell area and an increase in cell aspect ratio, in microgravity conditions. No significant difference was observed in cell angle of rotation at different time measurements. Also, in measuring viability of these cells using MTT test it was found that microgravity reduces viability of stem cells, considerably. <br>
<strong>Conclusion:</strong> Microgravity conditions have a considerable impact on morphology of MSCs. Furthermore, viability of MSCs decreased signi ficantly after 48 h, under microgravity conditions.</div>
Cell viability, Mesenchymal stem cell, Rat, Microgravity
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30
http://anatomyjournal.ir/browse.php?a_code=A-10-183-1&slc_lang=en&sid=1
Samira
Monfaredi
monfaredisamira@gmail.com
10031947532846003385
10031947532846003385
No
Department of Anatomical Sciences, Faculty of Medicine, Qazvin Branch, Islamic Azad University, Qazvin, Iran.
Shahram
Darabi
Shahram2005d@yahoo.com
10031947532846003386
10031947532846003386
No
Department of Anatomical Sciences, Faculty of Medicine, Qazvin Branch, Islamic Azad University, Qazvin, Iran.
Reza
Ahadi
Rezaahadi1353@gmail.com
10031947532846003387
10031947532846003387
No
Department of Anatomical Sciences, Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
Farzad
Rajaei
research.rasoul@gmail.com
10031947532846003388
10031947532846003388
Yes
Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.