AU - Nabiuni, Mohammad AU - Ganji, Roya AU - Bahadori, Mohammadhadi TI - Melatonin Impact on In Vitro Development of Mouse Preantral Follicles and Oocyte Maturation PT - JOURNAL ARTICLE TA - ASJ JN - ASJ VO - 10 VI - 1 IP - 1 4099 - http://anatomyjournal.ir/article-1-31-en.html 4100 - http://anatomyjournal.ir/article-1-31-en.pdf SO - ASJ 1 AB  - Introduction: Melatonin acts as an indirect antioxidant and is a powerful direct free radical scavenger and direct responses to melatonin in the gonads are detected. This study aims to investigate the influence of different doses of melatonin on preantral follicle development and oogenesis of in vitro cultured mouse ovarian follicles. Materials and Methods: Preantral follicles with diameters of 150–175 μm were mechanically isolated from NMRI mouse ovaries. Follicles were cultured in droplets of α-minimal essential medium (α-MEM) supplemented with 5% FBS, 100 mIU/ml rhFSH, 1% ITS, 100 IU/ml penicillin and 100 μg/ml streptomycin in conjunction with varying doses of melatonin (0, 1, 10, 100 nM and 100, 500 pM) for six days. On day six, in vitro ovulation was induced by the addition of hCG/rEGF to the culture medium and after 16-20 h the maturation state of the oocytes was assessed. Results: There was a significant (P CP - IRAN IN - LG - eng PB - ASJ PG - 7 PT - Original YR - 2013