Acquaintance with the different anatomical variations of the arterial supply of the gallbladder is of great importance in hepatobiliary surgical procedures. A rare variation of the hepatobiliary arterial system was found during anatomical dissection of a female Iranian cadaver. Two cystic arteries were present, the first arising directly from the right hepatic artery and the second from the gastroduodenal artery. Knowledge of the different anatomical variations of the arterial supply of the gallbladder is of great importance in hepatobiliary surgical procedures.