Volume 21, Issue 1 (Winter & Spring-InPress 2024)
ASJ 2024, 21(1): 0-0 |
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Moghani A, Azemati F, Hosseini S M, Jalali Kondori B. Effect of Glycyrrhizic acid and Curcumin on Wound Healing in Streptozotocin Induced Diabetic Mice. ASJ 2024; 21 (1)
URL: http://anatomyjournal.ir/article-1-632-en.html
URL: http://anatomyjournal.ir/article-1-632-en.html
1- Student Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran
2- Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3- Medicine, Quran and Hadith Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
4- Department of Anatomical Sciences, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
2- Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3- Medicine, Quran and Hadith Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
4- Department of Anatomical Sciences, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract: (271 Views)
Introduction: Diabetes Mellitus is one of the most common metabolic diseases in the world, which is associated with insulin resistance and dysfunction of pancreatic beta cells. One of the most serious complications of this disease is vascular disorders and diabetic ulcers, especially at the end of the patient's lower limbs. The aim of this study is to investigate the effects of the combination of curcumin and glycyrrhizic acid on the healing of diabetic wounds in mice.
Materials and Methods: In this study, to induce type 2 diabetes, animals were fed a high-fat diet (HFD) with a total caloric value of 4900 kcal/kg for 21 days, followed by a single dose of streptozotocin (35 mg/kg) to it was injected intraperitoneally. Serum glucose and insulin levels were measured using a glucometer and an ELISA kit, respectively. Histopathological examinations were also performed using hematoxylin and eosin and Masson's trichrome staining methods. Results: macroscopic evaluation of the wound showed; that the average speed of wound closure in the control group was much lower than the treatment groups. In the treatment group with silver sulfadiazine, glycyrrhizic acid and curcumin, the wound surface size decreased compared to the control group. The results of histopathological studies also showed that re-epithelization and granulation at the diabetic wound site increased in the treatment groups with glycyrrhizic acid and curcumin as well as silver sulfadiazine, but contrary to expectations, the rate of regeneration as well as collagen formation in the glycyrrhizic acid combined treatment group and curcumin did not have synergistic effects. Conclusion: The use of glycyrrhizic acid and curcumin separately leads to an increase in the speed of wound closure, and by causing a delay in the inflammation process and increasing the speed of epidermal regeneration, it accelerates the healing of diabetic wounds in mice.
Materials and Methods: In this study, to induce type 2 diabetes, animals were fed a high-fat diet (HFD) with a total caloric value of 4900 kcal/kg for 21 days, followed by a single dose of streptozotocin (35 mg/kg) to it was injected intraperitoneally. Serum glucose and insulin levels were measured using a glucometer and an ELISA kit, respectively. Histopathological examinations were also performed using hematoxylin and eosin and Masson's trichrome staining methods. Results: macroscopic evaluation of the wound showed; that the average speed of wound closure in the control group was much lower than the treatment groups. In the treatment group with silver sulfadiazine, glycyrrhizic acid and curcumin, the wound surface size decreased compared to the control group. The results of histopathological studies also showed that re-epithelization and granulation at the diabetic wound site increased in the treatment groups with glycyrrhizic acid and curcumin as well as silver sulfadiazine, but contrary to expectations, the rate of regeneration as well as collagen formation in the glycyrrhizic acid combined treatment group and curcumin did not have synergistic effects. Conclusion: The use of glycyrrhizic acid and curcumin separately leads to an increase in the speed of wound closure, and by causing a delay in the inflammation process and increasing the speed of epidermal regeneration, it accelerates the healing of diabetic wounds in mice.
Type of Study: Original |
Subject:
Histology
Received: 2025/02/17 | Accepted: 2025/04/7 | Published: 2024/04/2
Received: 2025/02/17 | Accepted: 2025/04/7 | Published: 2024/04/2
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