Volume 9, Issue 37 (Winter 2012)                   ASJ 2012, 9(37): 270-278 | Back to browse issues page

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Moghani Ghoroghi F, Nobakht M, Esmaeilzade B, Bakhtiyari M, Hejazian L B. Effect of Neurotrophin-3 on Differentiation of Rat Hair Follicle Stem Cells into Neural Like Cells. ASJ 2012; 9 (37) :270-278
URL: http://anatomyjournal.ir/article-1-544-en.html
Effect of Neurotrophin-3 on Differentiation of Rat Hair Follicle Stem Cells into Neural Like Cells
Fatemeh Moghani Ghoroghi , Maliheh Nobakht 1, Banfsheh Esmaeilzade , Mehrdad Bakhtiyari , Leila Beygom Hejazian
Abstract:   (617 Views)
Purpose: The aim of this study was to evaluate the effect of NT-3 on the decrease of the differentiation time of bulge stem cells of rat hair follicle from neuron like cells.Materials and Methods: The bulge region of the rat whisker was isolated from and cultured in DMEM/F12 supplemented with epidermal growth factor (EGF) in 3 groups for 7, 8 and 9 days .Then 10 ng/ml NT-3 was added to each group for 3 days. Finally, cell differentiation was assessed by immunocytochemistry using βⅢ-tubulin antibody and the result was compared with control groups.Results: According to our results hair follicle bulge stem cells expressed CD34 and Nestin in 7-9 and 10-12 first days after cultivation respectively. By using NT-3 duration differentiation process the cells showed positive reaction to βⅢ-tubulin antibody.Conclusion: The results indicate that NT-3 can affect on differentiation speed even in less than 10 days
Keywords: Bulge of Hair Follicle, Cd34, Nestin, Nt-3, ΒⅢ-Tubulin
Full-Text [PDF 444 kb]   (284 Downloads)    
Type of Study: Original | Subject: Morphometry
Received: 2021/12/31 | Accepted: 2012/02/21 | Published: 2012/02/21
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