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Purpose: To improve the development of frozen mouse embryos by adding fibroblast growth factors (FGF) and hepatocytes (HGF) to the culture medium. Materials and Methods: Two-cell mouse embryos obtained from the fallopian tube and frozen by cryotopic method were treated in T6 medium with a dose of 20 ng / ml FGF and 20 ng / ml HGF. The results of their culture were compared with control groups and the rate of embryonic development was evaluated.Results: The results showed that the rate of cleavage and development of vitrified and non-vitrified mouse embryos in the culture medium by adding growth factors was significantly increased compared to the control group (p <0.01). There was also a significant difference in the rate of morula development between vitrified and non-vitrified embryos treated with growth factors (p <0.05), but this difference was not observed in the rate of blastocyst formation (p> 0.05). Conclusion: The results of this study show that the addition of growth factors in the culture medium improves the pre-implantation development of mouse embryos after vitrification.

Keywords: Growth Factors, Pre-implantation Development, Vitrification

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