Volume 8, Issue 32 And 33 (Autumn & Winter 2010)                   ASJ 2010, 8(32 And 33): 164-168 | Back to browse issues page

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Asgari Z, Ramezani M, Bahadori M H, Ahmadi Jalali Moghaddam M. The Evaluation of Melatonin Effect on In-Vitro Development of Mouse Preimplantation Embryos. ASJ. 2010; 8 (32 and 33) :164-168
URL: http://anatomyjournal.ir/article-1-520-en.html
Abstract:   (266 Views)
Purpose: Melatonin promotes in-vitro embryo development in different species. This study studied the effects of melatonin on in-vitro mouse preimplantation embryo development. Materials and Methods: Two-cell embryos were obtained from oviduct of 6-8 weeks female NMRI mice 48 hours after administration of an intra-peritoneal injection of 5 IU/ml pregnant mares’ serum gonadotrophin and subsequent 5 IU/ml human chorionic gonadotrophin (ip). Embryos were cultured in T6 culture medium supplemented with different dosages of melatonin {0 (control group), 100×10-6 M, 10×10-6 M, 1×10-6 M, 100×10-9 M and 10×10-9 M (1-5 treatment groups)}. With due attention to percent of embryos in different stages, the rate of development of embryos was assessed using of invert microscope. It is also compared to control group. Results: The results showed that the rate of cleavage and development of mouse the embryos to blastocyst stage increased significantly in the development culture medium supplemented with 10 and 100 nM/mg of melatonin in comparison to control group (p<0.001). Conclusion: The results of this study demonstrate that, enriching the culture medium with melatonin, improve mouse preimplantation development.
     
Type of Study: Original | Subject: Morphometry
Received: 2021/12/30 | Accepted: 2010/11/7 | Published: 2010/11/7

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