Abstract: (688 Views)
Purpose: The aim of this research was to study the effects of different doses of LIF on GVBD and MII development rate and cumulus expansion. Materials and Methods: Immature mice superovulated with HMG and GV oocytes obtained from ovary 48 hours later. The GV oocytes were cultured in TCM199 with 100, 500 and 1000 IU /ml LIF. Cumulus expansions were evaluated with two examiners and numbers of MII oocytes were recorded. For denuding the oocytes hyaloronidase was used.Results: Our results showed that the rate of GVBD and MII development increased in groups with LIF compared with control group. Rate of MII development with 1000 IU/ml LIF was significantly higherthan that of control group (P<0.05). Cumulus expansion in group with 1000 IU/ml LIF improved significantly compared with control group (p<0.05). Conclusion: Our results showed that LIF could improve IVM rate in dose dependant. Also cumulus expansion improved in group with LIF and increased oocyte quality.
Type of Study:
Original |
Subject:
Morphometry Received: 2021/12/26 | Accepted: 2009/07/19 | Published: 2009/07/19