Volume 2, Issue 3 (Autumn 2004)
ASJ 2004, 2(3): 33-39 |
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1- Hematology Department, Tarbiat Modarres University, Tehran, Iran.
Abstract: (800 Views)
Purpose: This study was carried out to isolate mesenchymal stem cells from full-term cord blood and to differentiate these cells into osteoblastic cells.
Materials and Methods: It this study, mesenchymal stem cells were isolated from cord blood and expanded by being cultured in the flask several times. First, the cord blood monoeuc1earcells were separated by ficoll - Hypac before they were cultured in culture media; the medium was exchanged every week Next, the adherent cells were collected and expanded. The cord- blood mesenchymal stem cells were differentiated into osteoblastic cells by dexamethasone, ascorbic acid and b-glycerol phosphate.
Results: The expanded cells were similar to fibroblastic cells, and alkaline phosphates activity was low. These cells were differentiated after 12 days' induction and showed an enhanced activity in alkaline phosphates.
Conclusion: The results showed that cord-blood mesenchymal stem cells could be a substitution source for bone-marrow mesenchymal stem cells and that they could be used in researches and clinical studies.
Materials and Methods: It this study, mesenchymal stem cells were isolated from cord blood and expanded by being cultured in the flask several times. First, the cord blood monoeuc1earcells were separated by ficoll - Hypac before they were cultured in culture media; the medium was exchanged every week Next, the adherent cells were collected and expanded. The cord- blood mesenchymal stem cells were differentiated into osteoblastic cells by dexamethasone, ascorbic acid and b-glycerol phosphate.
Results: The expanded cells were similar to fibroblastic cells, and alkaline phosphates activity was low. These cells were differentiated after 12 days' induction and showed an enhanced activity in alkaline phosphates.
Conclusion: The results showed that cord-blood mesenchymal stem cells could be a substitution source for bone-marrow mesenchymal stem cells and that they could be used in researches and clinical studies.
Type of Study: Original |
Subject:
Morphometry
Received: 2021/12/26 | Accepted: 2004/10/19 | Published: 2004/10/19
Received: 2021/12/26 | Accepted: 2004/10/19 | Published: 2004/10/19
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