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Volume 2, Issue 3 (Autumn 2004)
ASJ 2004, 2(3): 13-19 |
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Ansari S, Kazemi B, Bandeh Pour M, Bayat M, Sadeghi Y, Rezaei F S. Effect of One Time Irradiation of Uvb Non Keratinocyte Growth Factor Gene Expression in Mice. ASJ 2004; 2 (3) :13-19
URL: http://anatomyjournal.ir/article-1-383-en.html
URL: http://anatomyjournal.ir/article-1-383-en.html
Simin Ansari 
, Bahram Kazemi , Mozhgan Bandeh Pour , Mohammad Bayat 1, Yoosef Sadeghi , Fatemeh Sadat Rezaei
, Bahram Kazemi , Mozhgan Bandeh Pour , Mohammad Bayat 1, Yoosef Sadeghi , Fatemeh Sadat Rezaei
1- Cellular And Molecular Biology Research Center, Shaheed Beheshti Medical Science University, Tehran, Iran.
Abstract: (848 Views)
Purpose: Skin is continuously exposed to many hazardous environmental factors such as ultraviolet radiation (UV). Many investigations have been shown to cause skin damages. The aim of present research was to study the effect of a single time of UVB radiation on the expression pattern of keratinocyte growth factor (KGF) gene in mice.
Materials and Methods: UVB (30 mJ/cm2 and 50 mJ/cm2) were radiated to the dorsum skin of two experimental groups of 30 Balb/C mice one time respectively. Skin samples were extracted from the mice three steps immediately after UVB exposure, two hours afte UBV exposure, and six hours UVB exposur. There were not any differences between experimental and control (n:15) groups in expection of UVB radiation, RNA of the skin samples were extracted and reverse-transcription polymerase chain reaction (RT-PCR) and nested PCR successively were used to amplify the KGF gene. Gene expression was revealed using agarose gel electerophorsis of products and were observed by a UV transilluminator instrument. Data were analysed with Fisher exact test method.
Results: The specific amplified DNA band of KGF gene were observed in all samples of conrol group, but there were not the band in any samples of experimental groups.
Conclusion: It seems that one time UVB radiation with 30 mJ/cm2 and 50 mJ/cm2 doses could abolish KGF gene expression in mice.
Materials and Methods: UVB (30 mJ/cm2 and 50 mJ/cm2) were radiated to the dorsum skin of two experimental groups of 30 Balb/C mice one time respectively. Skin samples were extracted from the mice three steps immediately after UVB exposure, two hours afte UBV exposure, and six hours UVB exposur. There were not any differences between experimental and control (n:15) groups in expection of UVB radiation, RNA of the skin samples were extracted and reverse-transcription polymerase chain reaction (RT-PCR) and nested PCR successively were used to amplify the KGF gene. Gene expression was revealed using agarose gel electerophorsis of products and were observed by a UV transilluminator instrument. Data were analysed with Fisher exact test method.
Results: The specific amplified DNA band of KGF gene were observed in all samples of conrol group, but there were not the band in any samples of experimental groups.
Conclusion: It seems that one time UVB radiation with 30 mJ/cm2 and 50 mJ/cm2 doses could abolish KGF gene expression in mice.
Keywords: Keratinocyte growth factor, Ultraviolet radiation, Mouse, Nested polymerase chain reaction
Type of Study: Original |
Subject:
Morphometry
Received: 2021/12/26 | Accepted: 2004/10/19 | Published: 2004/10/19
Received: 2021/12/26 | Accepted: 2004/10/19 | Published: 2004/10/19
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