Anatomical Sciences Journal
مجله علوم تشریح
ASJ
Basic Sciences
http://anatomyjournal.ir
1
admin
17286158
17286158
en
jalali
1393
11
1
gregorian
2015
2
1
12
1
online
1
fulltext
en
Studying of the Expression of BAX and BCL-2 Genes in Men with Sperm DNA Fragmentation Based on DFI and Comet Assay
Original
Original
<p dir="ltr" style="text-align: justify"><strong>Introduction:</strong> The amount of expression of BAX and BCL-2 genes in infertile men’s sperm as well as its association with sperm parameters and DNA fragmentation index is an issue which has not been studied yet. In this research, it is assumed that up-regulation of BAX and downregulation of BCL-2 are directly associated with sperm DNA fragmentation.<br>
<strong>Methods: </strong>After obtaining semen samples from the patients by using gradient centrifugation method, the semen samples were centrifuged using gradient method in order to obtain pure sperm. Sperm is divided into three parts based on which flow cytometry, Real Time-PCR and Comet Assay techniques were conducted. After extracting RNA and producing cDNA, the<br>
amount of expression of BAX and BCL-2 genes was measured using Real Time-PCR. The amount of sperm DNA fragmentation was measured using flow cytometry and Comet Assay techniques. Based on the amount of DNA fragmentation index (DFI), samples were divided into the two groups of control (DFI<30) and DNA fragmentation (DFI&ge30). Using WHO criteria, sperm parameters (morphology and motility) were evaluated.<br>
<strong>Results:</strong> This study showed that the amount of expression of BAX in the DNA fragmentation group was not significant compared to the control group but the expression of BCL-2 gene decreased significantly (P<0.05). Also, in many cases, there was a significant difference between the two groups in terms of parameters of sperm DNA fragmentation and morphological parameters (P<0.05).<br>
<strong>Conclusion:</strong> This study showed that reduction of expression of BCL-2 increases sperm DNA damage and this result can be helpful for therapeutic purposes.</p>
Sperm DNA damage, BAX, BCL-2
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http://anatomyjournal.ir/browse.php?a_code=A-10-80-1&slc_lang=en&sid=1
Hossein
Taheri
1003194753284600925
1003194753284600925
No
Organization
Mohammad
Salehi
msalehi78@gmail.com
1003194753284600926
1003194753284600926
Yes
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences and Health Services, Tehran, Iran.
Tarlan
Eslami Arshaghi
1003194753284600927
1003194753284600927
No
Organization
Majid
Mosahebi
1003194753284600928
1003194753284600928
No
Organization